Experiment: Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14610 and SGL_RS14620 are separated by 306 nucleotides SGL_RS14620 and SGL_RS14625 are separated by 43 nucleotides SGL_RS14625 and SGL_RS14630 are separated by 198 nucleotides
SGL_RS14610: SGL_RS14610 - RNA polymerase sigma factor SigC, at 2,731,560 to 2,732,774
_RS14610
SGL_RS14620: SGL_RS14620 - CoB--CoM heterodisulfide reductase iron-sulfur subunit B family protein, at 2,733,081 to 2,733,986
_RS14620
SGL_RS14625: SGL_RS14625 - YggT family protein, at 2,734,030 to 2,734,323
_RS14625
SGL_RS14630: SGL_RS14630 - pentapeptide repeat-containing protein, at 2,734,522 to 2,735,301
_RS14630
Position (kb)
2733
2734 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2732.134 kb on + strand, within SGL_RS14610 at 2732.142 kb on - strand, within SGL_RS14610 at 2732.161 kb on + strand, within SGL_RS14610 at 2732.219 kb on - strand, within SGL_RS14610 at 2732.478 kb on + strand, within SGL_RS14610 at 2732.865 kb on + strand at 2733.058 kb on + strand at 2733.141 kb on + strand at 2733.167 kb on + strand at 2733.470 kb on + strand, within SGL_RS14620 at 2733.537 kb on + strand, within SGL_RS14620 at 2733.649 kb on + strand, within SGL_RS14620 at 2733.650 kb on - strand, within SGL_RS14620 at 2733.694 kb on + strand, within SGL_RS14620 at 2733.795 kb on + strand, within SGL_RS14620 at 2733.796 kb on - strand, within SGL_RS14620 at 2733.855 kb on + strand, within SGL_RS14620 at 2733.855 kb on + strand, within SGL_RS14620 at 2733.870 kb on + strand, within SGL_RS14620 at 2733.878 kb on - strand, within SGL_RS14620 at 2733.890 kb on + strand, within SGL_RS14620 at 2733.978 kb on - strand at 2734.062 kb on - strand, within SGL_RS14625 at 2734.218 kb on + strand, within SGL_RS14625 at 2734.297 kb on - strand at 2734.447 kb on + strand at 2734.449 kb on + strand at 2734.449 kb on + strand at 2734.457 kb on - strand at 2734.526 kb on + strand at 2734.803 kb on - strand, within SGL_RS14630 at 2734.808 kb on - strand, within SGL_RS14630 at 2734.822 kb on + strand, within SGL_RS14630 at 2734.864 kb on + strand, within SGL_RS14630 at 2734.889 kb on - strand, within SGL_RS14630 at 2734.910 kb on + strand, within SGL_RS14630
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with TES 20 mM TES pH8 but diluted to prevent light attenuation remove 2,732,134 + SGL_RS14610 0.47 -0.4 2,732,142 - SGL_RS14610 0.48 -0.6 2,732,161 + SGL_RS14610 0.49 -0.1 2,732,219 - SGL_RS14610 0.54 +0.3 2,732,478 + SGL_RS14610 0.76 +0.1 2,732,865 + -0.3 2,733,058 + -4.0 2,733,141 + -3.7 2,733,167 + -4.5 2,733,470 + SGL_RS14620 0.43 -3.2 2,733,537 + SGL_RS14620 0.50 -4.4 2,733,649 + SGL_RS14620 0.63 -3.8 2,733,650 - SGL_RS14620 0.63 -4.7 2,733,694 + SGL_RS14620 0.68 -1.5 2,733,795 + SGL_RS14620 0.79 -5.8 2,733,796 - SGL_RS14620 0.79 -4.3 2,733,855 + SGL_RS14620 0.85 -2.7 2,733,855 + SGL_RS14620 0.85 -4.2 2,733,870 + SGL_RS14620 0.87 -3.2 2,733,878 - SGL_RS14620 0.88 -3.6 2,733,890 + SGL_RS14620 0.89 -3.2 2,733,978 - -3.6 2,734,062 - SGL_RS14625 0.11 -0.2 2,734,218 + SGL_RS14625 0.64 -0.7 2,734,297 - -0.5 2,734,447 + +0.5 2,734,449 + -0.2 2,734,449 + +0.3 2,734,457 - +0.1 2,734,526 + -0.0 2,734,803 - SGL_RS14630 0.36 +0.7 2,734,808 - SGL_RS14630 0.37 -0.6 2,734,822 + SGL_RS14630 0.38 +0.3 2,734,864 + SGL_RS14630 0.44 +0.2 2,734,889 - SGL_RS14630 0.47 -0.2 2,734,910 + SGL_RS14630 0.50 -0.2
Or see this region's nucleotide sequence