Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS11700 and SGL_RS11705 are separated by 226 nucleotides SGL_RS11705 and SGL_RS18930 are separated by 111 nucleotides SGL_RS18930 and SGL_RS11715 are separated by 161 nucleotides SGL_RS11715 and SGL_RS11720 are separated by 4 nucleotides SGL_RS11720 and SGL_RS11725 are separated by 27 nucleotides
SGL_RS11700: SGL_RS11700 - 2OG-Fe(II) oxygenase, at 2,082,864 to 2,083,916
_RS11700
SGL_RS11705: SGL_RS11705 - DUF2281 domain-containing protein, at 2,084,143 to 2,084,370
_RS11705
SGL_RS18930: SGL_RS18930 - PIN domain-containing protein, at 2,084,482 to 2,084,760
_RS18930
SGL_RS11715: SGL_RS11715 - BrnT family toxin, at 2,084,922 to 2,085,206
_RS11715
SGL_RS11720: SGL_RS11720 - hypothetical protein, at 2,085,211 to 2,085,417
_RS11720
SGL_RS11725: SGL_RS11725 - hypothetical protein, at 2,085,445 to 2,085,684
_RS11725
Position (kb)
2084
2085 Strain fitness (log2 ratio)
-1
0
1 at 2083.934 kb on + strand at 2084.038 kb on + strand at 2084.070 kb on + strand at 2084.073 kb on - strand at 2084.719 kb on + strand, within SGL_RS18930 at 2085.458 kb on + strand at 2085.581 kb on + strand, within SGL_RS11725 at 2085.715 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 2,083,934 + -0.7 2,084,038 + -0.3 2,084,070 + +0.1 2,084,073 - +0.0 2,084,719 + SGL_RS18930 0.85 -1.1 2,085,458 + -0.1 2,085,581 + SGL_RS11725 0.57 -0.4 2,085,715 - -0.0
Or see this region's nucleotide sequence