Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09860 and SGL_RS09865 are separated by 213 nucleotides SGL_RS09865 and SGL_RS09870 are separated by 79 nucleotides SGL_RS09870 and SGL_RS09875 are separated by 71 nucleotides
SGL_RS09860: SGL_RS09860 - carotenoid biosynthesis protein, at 1,695,886 to 1,696,797
_RS09860
SGL_RS09865: SGL_RS09865 - cytochrome c oxidase subunit II, at 1,697,011 to 1,697,913
_RS09865
SGL_RS09870: SGL_RS09870 - pantothenate kinase, at 1,697,993 to 1,698,766
_RS09870
SGL_RS09875: SGL_RS09875 - glycosyltransferase, at 1,698,838 to 1,699,980
_RS09875
Position (kb)
1697
1698 Strain fitness (log2 ratio)
-1
0
1 at 1696.044 kb on + strand, within SGL_RS09860 at 1696.178 kb on + strand, within SGL_RS09860 at 1696.178 kb on + strand, within SGL_RS09860 at 1696.529 kb on + strand, within SGL_RS09860 at 1696.713 kb on - strand at 1696.723 kb on - strand at 1696.901 kb on + strand at 1696.915 kb on + strand at 1697.058 kb on - strand at 1697.073 kb on + strand at 1697.208 kb on + strand, within SGL_RS09865 at 1697.223 kb on - strand, within SGL_RS09865 at 1697.272 kb on + strand, within SGL_RS09865 at 1697.284 kb on + strand, within SGL_RS09865 at 1697.344 kb on + strand, within SGL_RS09865 at 1697.556 kb on - strand, within SGL_RS09865 at 1697.583 kb on + strand, within SGL_RS09865 at 1697.818 kb on + strand, within SGL_RS09865 at 1697.832 kb on - strand at 1697.865 kb on - strand at 1698.023 kb on + strand at 1698.048 kb on - strand at 1698.766 kb on + strand at 1698.775 kb on - strand at 1698.823 kb on - strand at 1698.877 kb on + strand at 1698.885 kb on - strand at 1698.885 kb on - strand at 1698.897 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 1,696,044 + SGL_RS09860 0.17 -1.1 1,696,178 + SGL_RS09860 0.32 -0.0 1,696,178 + SGL_RS09860 0.32 -0.1 1,696,529 + SGL_RS09860 0.71 +0.9 1,696,713 - +0.0 1,696,723 - -0.1 1,696,901 + +0.6 1,696,915 + +0.1 1,697,058 - +0.3 1,697,073 + -0.7 1,697,208 + SGL_RS09865 0.22 -0.1 1,697,223 - SGL_RS09865 0.23 -0.7 1,697,272 + SGL_RS09865 0.29 -0.8 1,697,284 + SGL_RS09865 0.30 -0.5 1,697,344 + SGL_RS09865 0.37 -0.1 1,697,556 - SGL_RS09865 0.60 -0.4 1,697,583 + SGL_RS09865 0.63 +0.0 1,697,818 + SGL_RS09865 0.89 -0.2 1,697,832 - -0.1 1,697,865 - -0.1 1,698,023 + -0.4 1,698,048 - +0.8 1,698,766 + +0.6 1,698,775 - -0.2 1,698,823 - +0.2 1,698,877 + +0.1 1,698,885 - +0.2 1,698,885 - +0.4 1,698,897 + +0.2
Or see this region's nucleotide sequence