Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09140 and SGL_RS09145 are separated by 218 nucleotides SGL_RS09145 and SGL_RS09150 are separated by 36 nucleotides SGL_RS09150 and SGL_RS09155 are separated by 308 nucleotides SGL_RS09155 and SGL_RS09160 are separated by 441 nucleotides
SGL_RS09140: SGL_RS09140 - IS701 family transposase, at 1,554,855 to 1,555,645
_RS09140
SGL_RS09145: SGL_RS09145 - Uma2 family endonuclease, at 1,555,864 to 1,556,438
_RS09145
SGL_RS09150: SGL_RS09150 - acetolactate synthase large subunit, at 1,556,475 to 1,558,118
_RS09150
SGL_RS09155: SGL_RS09155 - riboflavin synthase subunit alpha, at 1,558,427 to 1,558,627
_RS09155
SGL_RS09160: SGL_RS09160 - thioredoxin family protein, at 1,559,069 to 1,559,611
_RS09160
Position (kb)
1556
1557
1558
1559 Strain fitness (log2 ratio)
-2
-1
0
1 at 1555.895 kb on + strand at 1555.924 kb on - strand, within SGL_RS09145 at 1555.967 kb on - strand, within SGL_RS09145 at 1556.016 kb on + strand, within SGL_RS09145 at 1556.045 kb on + strand, within SGL_RS09145 at 1556.164 kb on + strand, within SGL_RS09145 at 1556.300 kb on - strand, within SGL_RS09145 at 1556.326 kb on - strand, within SGL_RS09145 at 1556.458 kb on - strand at 1556.550 kb on + strand at 1556.562 kb on + strand at 1556.577 kb on - strand at 1556.857 kb on - strand, within SGL_RS09150 at 1556.895 kb on + strand, within SGL_RS09150 at 1557.400 kb on + strand, within SGL_RS09150 at 1557.407 kb on - strand, within SGL_RS09150 at 1557.414 kb on + strand, within SGL_RS09150 at 1557.807 kb on + strand, within SGL_RS09150 at 1558.222 kb on + strand at 1558.266 kb on + strand at 1558.274 kb on - strand at 1558.669 kb on + strand at 1558.937 kb on - strand at 1559.104 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 1,555,895 + +0.3 1,555,924 - SGL_RS09145 0.10 +0.4 1,555,967 - SGL_RS09145 0.18 -0.8 1,556,016 + SGL_RS09145 0.26 -1.1 1,556,045 + SGL_RS09145 0.31 +0.1 1,556,164 + SGL_RS09145 0.52 +1.1 1,556,300 - SGL_RS09145 0.76 -0.5 1,556,326 - SGL_RS09145 0.80 -0.4 1,556,458 - -0.1 1,556,550 + -2.3 1,556,562 + -1.9 1,556,577 - -1.9 1,556,857 - SGL_RS09150 0.23 -0.9 1,556,895 + SGL_RS09150 0.26 -1.8 1,557,400 + SGL_RS09150 0.56 -1.8 1,557,407 - SGL_RS09150 0.57 -2.5 1,557,414 + SGL_RS09150 0.57 -1.5 1,557,807 + SGL_RS09150 0.81 -1.9 1,558,222 + -0.1 1,558,266 + -0.1 1,558,274 - -0.2 1,558,669 + +0.2 1,558,937 - -0.3 1,559,104 + -0.0
Or see this region's nucleotide sequence