Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07670 and SGL_RS07675 are separated by 10 nucleotides SGL_RS07675 and SGL_RS07680 are separated by 204 nucleotides SGL_RS07680 and SGL_RS07685 are separated by 159 nucleotides
SGL_RS07670: SGL_RS07670 - riboflavin biosynthesis protein RibF, at 1,240,361 to 1,241,302
_RS07670
SGL_RS07675: SGL_RS07675 - HpsJ family protein, at 1,241,313 to 1,242,131
_RS07675
SGL_RS07680: SGL_RS07680 - DUF3143 domain-containing protein, at 1,242,336 to 1,242,605
_RS07680
SGL_RS07685: SGL_RS07685 - Calvin cycle protein CP12, at 1,242,765 to 1,242,989
_RS07685
Position (kb)
1241
1242
1243 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1240.351 kb on + strand at 1241.746 kb on - strand, within SGL_RS07675 at 1241.780 kb on - strand, within SGL_RS07675 at 1241.793 kb on - strand, within SGL_RS07675 at 1241.841 kb on + strand, within SGL_RS07675 at 1241.923 kb on + strand, within SGL_RS07675 at 1241.979 kb on - strand, within SGL_RS07675 at 1242.150 kb on - strand at 1242.271 kb on - strand at 1242.417 kb on + strand, within SGL_RS07680 at 1242.448 kb on - strand, within SGL_RS07680 at 1242.668 kb on - strand at 1242.791 kb on - strand, within SGL_RS07685 at 1242.791 kb on - strand, within SGL_RS07685 at 1243.010 kb on + strand at 1243.020 kb on + strand at 1243.020 kb on + strand at 1243.026 kb on + strand at 1243.054 kb on + strand at 1243.054 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 1,240,351 + -0.7 1,241,746 - SGL_RS07675 0.53 -2.7 1,241,780 - SGL_RS07675 0.57 -1.0 1,241,793 - SGL_RS07675 0.59 -2.3 1,241,841 + SGL_RS07675 0.64 -2.0 1,241,923 + SGL_RS07675 0.74 -2.2 1,241,979 - SGL_RS07675 0.81 -3.5 1,242,150 - -1.5 1,242,271 - +0.7 1,242,417 + SGL_RS07680 0.30 +0.2 1,242,448 - SGL_RS07680 0.41 +0.0 1,242,668 - -0.6 1,242,791 - SGL_RS07685 0.12 -2.0 1,242,791 - SGL_RS07685 0.12 -0.7 1,243,010 + -0.8 1,243,020 + +0.9 1,243,020 + -2.4 1,243,026 + -0.0 1,243,054 + -0.5 1,243,054 + -0.2
Or see this region's nucleotide sequence