Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04210 and SGL_RS04215 are separated by 88 nucleotides SGL_RS04215 and SGL_RS04220 are separated by 129 nucleotides SGL_RS04220 and SGL_RS04225 are separated by 443 nucleotides
SGL_RS04210: SGL_RS04210 - methyl-accepting chemotaxis protein, at 492,130 to 494,739
_RS04210
SGL_RS04215: SGL_RS04215 - MlaE family lipid ABC transporter permease subunit, at 494,828 to 495,619
_RS04215
SGL_RS04220: SGL_RS04220 - TatA/E family twin arginine-targeting protein translocase, at 495,749 to 496,006
_RS04220
SGL_RS04225: SGL_RS04225 - sensor domain-containing diguanylate cyclase, at 496,450 to 497,844
_RS04225
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 493.836 kb on + strand, within SGL_RS04210 at 493.836 kb on + strand, within SGL_RS04210 at 493.844 kb on - strand, within SGL_RS04210 at 493.941 kb on + strand, within SGL_RS04210 at 494.020 kb on - strand, within SGL_RS04210 at 494.079 kb on + strand, within SGL_RS04210 at 494.164 kb on + strand, within SGL_RS04210 at 494.263 kb on - strand, within SGL_RS04210 at 494.482 kb on + strand at 494.485 kb on - strand at 494.485 kb on - strand at 494.515 kb on - strand at 494.595 kb on + strand at 494.646 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.740 kb on + strand at 494.745 kb on + strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.748 kb on - strand at 494.946 kb on + strand, within SGL_RS04215 at 494.956 kb on - strand, within SGL_RS04215 at 495.038 kb on - strand, within SGL_RS04215 at 495.094 kb on - strand, within SGL_RS04215 at 495.272 kb on + strand, within SGL_RS04215 at 495.280 kb on - strand, within SGL_RS04215 at 495.400 kb on + strand, within SGL_RS04215 at 495.504 kb on - strand, within SGL_RS04215 at 495.532 kb on + strand, within SGL_RS04215 at 495.648 kb on + strand at 496.069 kb on + strand at 496.077 kb on - strand at 496.084 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 493,836 + SGL_RS04210 0.65 +0.2 493,836 + SGL_RS04210 0.65 -0.7 493,844 - SGL_RS04210 0.66 -0.1 493,941 + SGL_RS04210 0.69 +0.1 494,020 - SGL_RS04210 0.72 -0.6 494,079 + SGL_RS04210 0.75 +0.1 494,164 + SGL_RS04210 0.78 +0.4 494,263 - SGL_RS04210 0.82 -0.1 494,482 + +0.0 494,485 - +0.3 494,485 - +0.3 494,515 - -0.0 494,595 + -0.6 494,646 + -0.5 494,740 + +0.0 494,740 + +0.2 494,740 + -0.6 494,740 + -1.6 494,740 + +0.4 494,745 + -0.1 494,748 - -0.2 494,748 - -0.9 494,748 - -2.1 494,748 - -0.1 494,946 + SGL_RS04215 0.15 -2.4 494,956 - SGL_RS04215 0.16 -0.8 495,038 - SGL_RS04215 0.27 -2.8 495,094 - SGL_RS04215 0.34 -0.2 495,272 + SGL_RS04215 0.56 -2.8 495,280 - SGL_RS04215 0.57 -1.5 495,400 + SGL_RS04215 0.72 +1.3 495,504 - SGL_RS04215 0.85 -3.1 495,532 + SGL_RS04215 0.89 -1.2 495,648 + -0.1 496,069 + -0.4 496,077 - -0.1 496,084 - -0.6
Or see this region's nucleotide sequence