Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04020 and SGL_RS04025 are separated by 181 nucleotides SGL_RS04025 and SGL_RS04030 are separated by 110 nucleotides SGL_RS04030 and SGL_RS04035 are separated by 342 nucleotides
SGL_RS04020: SGL_RS04020 - hypothetical protein, at 454,712 to 456,793
_RS04020
SGL_RS04025: SGL_RS04025 - proton extrusion protein PcxA, at 456,975 to 458,297
_RS04025
SGL_RS04030: SGL_RS04030 - DUF4278 domain-containing protein, at 458,408 to 458,857
_RS04030
SGL_RS04035: SGL_RS04035 - ParA family protein, at 459,200 to 460,096
_RS04035
Position (kb) 456
457
458
459 Strain fitness (log2 ratio) -2
-1
0
1 at 455.999 kb on + strand, within SGL_RS04020 at 456.000 kb on - strand, within SGL_RS04020 at 456.038 kb on + strand, within SGL_RS04020 at 456.273 kb on + strand, within SGL_RS04020 at 456.273 kb on + strand, within SGL_RS04020 at 456.328 kb on - strand, within SGL_RS04020 at 456.380 kb on + strand, within SGL_RS04020 at 456.398 kb on - strand, within SGL_RS04020 at 456.464 kb on + strand, within SGL_RS04020 at 456.563 kb on - strand, within SGL_RS04020 at 456.621 kb on - strand at 456.621 kb on - strand at 456.699 kb on + strand at 456.777 kb on - strand at 457.205 kb on + strand, within SGL_RS04025 at 457.345 kb on - strand, within SGL_RS04025 at 457.346 kb on - strand, within SGL_RS04025 at 457.762 kb on - strand, within SGL_RS04025 at 457.916 kb on + strand, within SGL_RS04025 at 458.106 kb on + strand, within SGL_RS04025 at 458.106 kb on + strand, within SGL_RS04025 at 458.499 kb on - strand, within SGL_RS04030 at 458.654 kb on + strand, within SGL_RS04030 at 458.836 kb on + strand at 458.847 kb on + strand at 458.999 kb on - strand at 459.039 kb on + strand at 459.071 kb on - strand at 459.134 kb on + strand at 459.160 kb on + strand at 459.160 kb on + strand at 459.166 kb on - strand at 459.288 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 455,999 + SGL_RS04020 0.62 -0.4 456,000 - SGL_RS04020 0.62 -0.0 456,038 + SGL_RS04020 0.64 -0.0 456,273 + SGL_RS04020 0.75 -0.9 456,273 + SGL_RS04020 0.75 -0.1 456,328 - SGL_RS04020 0.78 -0.8 456,380 + SGL_RS04020 0.80 +0.7 456,398 - SGL_RS04020 0.81 +0.0 456,464 + SGL_RS04020 0.84 -0.4 456,563 - SGL_RS04020 0.89 -0.2 456,621 - +0.5 456,621 - +1.3 456,699 + +0.4 456,777 - +1.2 457,205 + SGL_RS04025 0.17 -1.2 457,345 - SGL_RS04025 0.28 -1.5 457,346 - SGL_RS04025 0.28 -2.0 457,762 - SGL_RS04025 0.59 -1.0 457,916 + SGL_RS04025 0.71 -0.8 458,106 + SGL_RS04025 0.85 -1.5 458,106 + SGL_RS04025 0.85 -0.8 458,499 - SGL_RS04030 0.20 -0.5 458,654 + SGL_RS04030 0.55 -0.1 458,836 + -0.9 458,847 + -1.7 458,999 - -0.6 459,039 + +0.9 459,071 - -0.3 459,134 + -0.0 459,160 + -0.4 459,160 + -0.4 459,166 - -0.2 459,288 - -0.4
Or see this region's nucleotide sequence