Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03560 and SGL_RS03565 are separated by 34 nucleotides SGL_RS03565 and SGL_RS03570 are separated by 50 nucleotides SGL_RS03570 and SGL_RS03575 are separated by 151 nucleotides SGL_RS03575 and SGL_RS19770 are separated by 163 nucleotides
SGL_RS03560: SGL_RS03560 - class I SAM-dependent methyltransferase, at 355,990 to 357,210
_RS03560
SGL_RS03565: SGL_RS03565 - DUF29 domain-containing protein, at 357,245 to 357,697
_RS03565
SGL_RS03570: SGL_RS03570 - Uma2 family endonuclease, at 357,748 to 358,608
_RS03570
SGL_RS03575: SGL_RS03575 - Uma2 family endonuclease, at 358,760 to 359,350
_RS03575
SGL_RS19770: SGL_RS19770 - DUF4160 domain-containing protein, at 359,514 to 359,663
_RS19770
Position (kb) 357
358
359 Strain fitness (log2 ratio) -2
-1
0
1 at 356.820 kb on - strand, within SGL_RS03560 at 356.949 kb on + strand, within SGL_RS03560 at 356.959 kb on - strand, within SGL_RS03560 at 357.035 kb on - strand, within SGL_RS03560 at 357.433 kb on - strand, within SGL_RS03565 at 357.474 kb on + strand, within SGL_RS03565 at 357.487 kb on - strand, within SGL_RS03565 at 358.350 kb on + strand, within SGL_RS03570 at 358.357 kb on + strand, within SGL_RS03570 at 358.518 kb on + strand, within SGL_RS03570 at 358.545 kb on + strand at 358.553 kb on - strand at 359.135 kb on - strand, within SGL_RS03575 at 359.477 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 356,820 - SGL_RS03560 0.68 +0.2 356,949 + SGL_RS03560 0.79 -0.1 356,959 - SGL_RS03560 0.79 -1.1 357,035 - SGL_RS03560 0.86 -0.3 357,433 - SGL_RS03565 0.42 +0.0 357,474 + SGL_RS03565 0.51 +0.2 357,487 - SGL_RS03565 0.53 +0.8 358,350 + SGL_RS03570 0.70 -1.8 358,357 + SGL_RS03570 0.71 -1.0 358,518 + SGL_RS03570 0.89 -0.6 358,545 + +0.1 358,553 - -1.5 359,135 - SGL_RS03575 0.63 +0.5 359,477 - -0.1
Or see this region's nucleotide sequence