Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03410 and SGL_RS03415 are separated by 40 nucleotides SGL_RS03415 and SGL_RS03420 are separated by 33 nucleotides SGL_RS03420 and SGL_RS03425 are separated by 83 nucleotides
SGL_RS03410: SGL_RS03410 - LL-diaminopimelate aminotransferase, at 328,006 to 329,181
_RS03410
SGL_RS03415: SGL_RS03415 - SH3 domain-containing protein, at 329,222 to 329,521
_RS03415
SGL_RS03420: SGL_RS03420 - UbiD family decarboxylase, at 329,555 to 331,060
_RS03420
SGL_RS03425: SGL_RS03425 - precorrin-3B C(17)-methyltransferase, at 331,144 to 333,027
_RS03425
Position (kb) 329
330
331
332 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 328.598 kb on + strand, within SGL_RS03410 at 328.603 kb on + strand, within SGL_RS03410 at 328.690 kb on + strand, within SGL_RS03410 at 328.698 kb on - strand, within SGL_RS03410 at 328.712 kb on + strand, within SGL_RS03410 at 328.720 kb on - strand, within SGL_RS03410 at 328.801 kb on + strand, within SGL_RS03410 at 328.822 kb on - strand, within SGL_RS03410 at 329.019 kb on - strand, within SGL_RS03410 at 329.053 kb on + strand, within SGL_RS03410 at 329.097 kb on - strand at 329.100 kb on + strand at 329.627 kb on + strand at 329.651 kb on + strand at 329.663 kb on + strand at 329.674 kb on - strand at 329.868 kb on - strand, within SGL_RS03420 at 330.016 kb on - strand, within SGL_RS03420 at 330.068 kb on + strand, within SGL_RS03420 at 330.330 kb on - strand, within SGL_RS03420 at 330.330 kb on - strand, within SGL_RS03420 at 330.547 kb on + strand, within SGL_RS03420 at 330.555 kb on - strand, within SGL_RS03420 at 330.783 kb on - strand, within SGL_RS03420 at 330.870 kb on - strand, within SGL_RS03420 at 330.900 kb on - strand, within SGL_RS03420 at 330.946 kb on - strand at 330.988 kb on + strand at 331.058 kb on - strand at 331.063 kb on - strand at 331.075 kb on - strand at 331.077 kb on + strand at 331.620 kb on - strand, within SGL_RS03425 at 331.870 kb on + strand, within SGL_RS03425 at 331.980 kb on - strand, within SGL_RS03425
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 328,598 + SGL_RS03410 0.50 +1.2 328,603 + SGL_RS03410 0.51 +0.0 328,690 + SGL_RS03410 0.58 +0.6 328,698 - SGL_RS03410 0.59 -0.1 328,712 + SGL_RS03410 0.60 -0.1 328,720 - SGL_RS03410 0.61 -0.2 328,801 + SGL_RS03410 0.68 +0.2 328,822 - SGL_RS03410 0.69 +0.0 329,019 - SGL_RS03410 0.86 +0.5 329,053 + SGL_RS03410 0.89 +0.0 329,097 - -0.6 329,100 + +0.0 329,627 + -0.8 329,651 + -2.6 329,663 + -0.1 329,674 - -1.3 329,868 - SGL_RS03420 0.21 -0.5 330,016 - SGL_RS03420 0.31 -1.2 330,068 + SGL_RS03420 0.34 +0.0 330,330 - SGL_RS03420 0.51 -3.3 330,330 - SGL_RS03420 0.51 -0.9 330,547 + SGL_RS03420 0.66 -1.1 330,555 - SGL_RS03420 0.66 -2.3 330,783 - SGL_RS03420 0.82 -1.7 330,870 - SGL_RS03420 0.87 -1.7 330,900 - SGL_RS03420 0.89 -1.4 330,946 - -1.3 330,988 + -0.7 331,058 - -0.0 331,063 - -0.6 331,075 - -1.8 331,077 + -0.5 331,620 - SGL_RS03425 0.25 -1.3 331,870 + SGL_RS03425 0.39 +0.3 331,980 - SGL_RS03425 0.44 -0.7
Or see this region's nucleotide sequence