Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03245 and SGL_RS03250 are separated by 81 nucleotides SGL_RS03250 and SGL_RS19555 are separated by 10 nucleotides SGL_RS19555 and SGL_RS03255 are separated by 208 nucleotides
SGL_RS03245: SGL_RS03245 - Fe(3+) ABC transporter substrate-binding protein, at 291,626 to 292,708
_RS03245
SGL_RS03250: SGL_RS03250 - tRNA (guanosine(37)-N1)-methyltransferase TrmD, at 292,790 to 293,485
_RS03250
SGL_RS19555: SGL_RS19555 - hypothetical protein, at 293,496 to 293,669
_RS19555
SGL_RS03255: SGL_RS03255 - ATP-dependent 6-phosphofructokinase, at 293,878 to 294,963
_RS03255
Position (kb) 292
293
294 Strain fitness (log2 ratio) -2
-1
0
1 at 291.820 kb on - strand, within SGL_RS03245 at 291.891 kb on - strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 291.898 kb on + strand, within SGL_RS03245 at 292.224 kb on + strand, within SGL_RS03245 at 292.230 kb on + strand, within SGL_RS03245 at 292.242 kb on - strand, within SGL_RS03245 at 292.248 kb on - strand, within SGL_RS03245 at 292.282 kb on - strand, within SGL_RS03245 at 292.307 kb on + strand, within SGL_RS03245 at 292.358 kb on - strand, within SGL_RS03245 at 292.371 kb on + strand, within SGL_RS03245 at 292.447 kb on + strand, within SGL_RS03245 at 292.471 kb on + strand, within SGL_RS03245 at 292.487 kb on + strand, within SGL_RS03245 at 292.489 kb on - strand, within SGL_RS03245 at 292.509 kb on + strand, within SGL_RS03245 at 292.659 kb on - strand at 292.661 kb on + strand at 292.669 kb on - strand at 292.669 kb on - strand at 292.781 kb on + strand at 292.895 kb on - strand, within SGL_RS03250 at 292.923 kb on + strand, within SGL_RS03250 at 293.154 kb on + strand, within SGL_RS03250 at 293.167 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.227 kb on - strand, within SGL_RS03250 at 293.330 kb on - strand, within SGL_RS03250 at 293.412 kb on + strand, within SGL_RS03250 at 293.585 kb on - strand, within SGL_RS19555 at 293.607 kb on - strand, within SGL_RS19555 at 293.698 kb on + strand at 293.859 kb on + strand at 293.986 kb on + strand at 294.065 kb on + strand, within SGL_RS03255 at 294.065 kb on + strand, within SGL_RS03255 at 294.087 kb on - strand, within SGL_RS03255 at 294.123 kb on - strand, within SGL_RS03255 at 294.226 kb on - strand, within SGL_RS03255 at 294.232 kb on + strand, within SGL_RS03255 at 294.245 kb on - strand, within SGL_RS03255 at 294.261 kb on + strand, within SGL_RS03255 at 294.318 kb on + strand, within SGL_RS03255
Per-strain Table
Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8 remove 291,820 - SGL_RS03245 0.18 -0.1 291,891 - SGL_RS03245 0.24 -0.4 291,898 + SGL_RS03245 0.25 -0.4 291,898 + SGL_RS03245 0.25 -0.1 292,224 + SGL_RS03245 0.55 -0.2 292,230 + SGL_RS03245 0.56 +0.1 292,242 - SGL_RS03245 0.57 +0.2 292,248 - SGL_RS03245 0.57 +0.2 292,282 - SGL_RS03245 0.61 +0.4 292,307 + SGL_RS03245 0.63 -0.5 292,358 - SGL_RS03245 0.68 +0.2 292,371 + SGL_RS03245 0.69 +0.0 292,447 + SGL_RS03245 0.76 -0.9 292,471 + SGL_RS03245 0.78 -0.3 292,487 + SGL_RS03245 0.80 -2.2 292,489 - SGL_RS03245 0.80 +0.2 292,509 + SGL_RS03245 0.82 -0.9 292,659 - +0.1 292,661 + +0.7 292,669 - +0.1 292,669 - -0.1 292,781 + +0.2 292,895 - SGL_RS03250 0.15 -1.4 292,923 + SGL_RS03250 0.19 -1.3 293,154 + SGL_RS03250 0.52 -0.5 293,167 - SGL_RS03250 0.54 -2.1 293,227 - SGL_RS03250 0.63 -0.3 293,227 - SGL_RS03250 0.63 -2.4 293,330 - SGL_RS03250 0.78 -1.3 293,412 + SGL_RS03250 0.89 -2.3 293,585 - SGL_RS19555 0.51 -0.4 293,607 - SGL_RS19555 0.64 +0.3 293,698 + +0.2 293,859 + +0.4 293,986 + +0.1 294,065 + SGL_RS03255 0.17 +0.2 294,065 + SGL_RS03255 0.17 +0.7 294,087 - SGL_RS03255 0.19 +0.1 294,123 - SGL_RS03255 0.23 -0.1 294,226 - SGL_RS03255 0.32 -0.1 294,232 + SGL_RS03255 0.33 -0.2 294,245 - SGL_RS03255 0.34 -0.1 294,261 + SGL_RS03255 0.35 +0.3 294,318 + SGL_RS03255 0.41 -0.8
Or see this region's nucleotide sequence