Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS00410

Experiment: Arg 10 mM as N source, buffered with 20 mM TES pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS00405 and SGL_RS00410 are separated by 93 nucleotidesSGL_RS00410 and SGL_RS00415 are separated by 71 nucleotidesSGL_RS00415 and SGL_RS00420 overlap by 61 nucleotides SGL_RS00405: SGL_RS00405 - DUF2267 domain-containing protein, at 87,601 to 88,020 _RS00405 SGL_RS00410: SGL_RS00410 - SDR family oxidoreductase, at 88,114 to 88,830 _RS00410 SGL_RS00415: SGL_RS00415 - metal-dependent hydrolase, at 88,902 to 89,759 _RS00415 SGL_RS00420: SGL_RS00420 - hypothetical protein, at 89,699 to 90,253 _RS00420 Position (kb) 88 89Strain fitness (log2 ratio) -1 0 1at 87.686 kb on + strand, within SGL_RS00405at 88.624 kb on + strand, within SGL_RS00410at 88.751 kb on - strand, within SGL_RS00410at 88.786 kb on - strandat 89.211 kb on + strand, within SGL_RS00415at 89.226 kb on + strand, within SGL_RS00415at 89.308 kb on - strand, within SGL_RS00415at 89.345 kb on - strand, within SGL_RS00415at 89.346 kb on + strand, within SGL_RS00415at 89.481 kb on - strand, within SGL_RS00415at 89.580 kb on + strand, within SGL_RS00415at 89.612 kb on + strand, within SGL_RS00415at 89.621 kb on - strand, within SGL_RS00415at 89.694 kb on + strandat 89.696 kb on + strandat 89.723 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Arg 10 mM as N source, buffered with 20 mM TES pH8
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87,686 + SGL_RS00405 0.20 +0.6
88,624 + SGL_RS00410 0.71 -1.1
88,751 - SGL_RS00410 0.89 -1.6
88,786 - +0.2
89,211 + SGL_RS00415 0.36 +0.0
89,226 + SGL_RS00415 0.38 -0.2
89,308 - SGL_RS00415 0.47 -0.2
89,345 - SGL_RS00415 0.52 +0.1
89,346 + SGL_RS00415 0.52 -0.8
89,481 - SGL_RS00415 0.67 +0.7
89,580 + SGL_RS00415 0.79 -0.2
89,612 + SGL_RS00415 0.83 -0.0
89,621 - SGL_RS00415 0.84 +0.2
89,694 + +0.0
89,696 + -0.3
89,723 - +0.1

Or see this region's nucleotide sequence