Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13080 and SGL_RS13085 are separated by 91 nucleotides SGL_RS13085 and SGL_RS19620 are separated by 105 nucleotides SGL_RS19620 and SGL_RS13090 are separated by 152 nucleotides
SGL_RS13080: SGL_RS13080 - rhodanese-related sulfurtransferase, at 2,406,130 to 2,406,966
_RS13080
SGL_RS13085: SGL_RS13085 - tRNA-Ala, at 2,407,058 to 2,407,130
_RS13085
SGL_RS19620: SGL_RS19620 - hypothetical protein, at 2,407,236 to 2,407,412
_RS19620
SGL_RS13090: SGL_RS13090 - urea ABC transporter ATP-binding protein UrtD, at 2,407,565 to 2,408,686
_RS13090
Position (kb)
2407
2408 Strain fitness (log2 ratio)
-1
0
1 at 2406.722 kb on + strand, within SGL_RS13080 at 2406.760 kb on + strand, within SGL_RS13080 at 2406.920 kb on + strand at 2406.928 kb on - strand at 2406.999 kb on + strand at 2407.394 kb on + strand, within SGL_RS19620 at 2407.507 kb on - strand at 2407.759 kb on + strand, within SGL_RS13090 at 2407.810 kb on + strand, within SGL_RS13090 at 2408.019 kb on + strand, within SGL_RS13090 at 2408.020 kb on - strand, within SGL_RS13090 at 2408.025 kb on - strand, within SGL_RS13090 at 2408.246 kb on + strand, within SGL_RS13090 at 2408.302 kb on + strand, within SGL_RS13090 at 2408.310 kb on - strand, within SGL_RS13090 at 2408.325 kb on + strand, within SGL_RS13090 at 2408.325 kb on - strand, within SGL_RS13090 at 2408.404 kb on + strand, within SGL_RS13090
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,406,722 + SGL_RS13080 0.71 +0.2 2,406,760 + SGL_RS13080 0.75 -1.6 2,406,920 + +0.3 2,406,928 - -0.3 2,406,999 + +0.0 2,407,394 + SGL_RS19620 0.89 -0.9 2,407,507 - -0.2 2,407,759 + SGL_RS13090 0.17 -0.1 2,407,810 + SGL_RS13090 0.22 -0.2 2,408,019 + SGL_RS13090 0.40 -0.6 2,408,020 - SGL_RS13090 0.41 -0.9 2,408,025 - SGL_RS13090 0.41 +0.7 2,408,246 + SGL_RS13090 0.61 +0.0 2,408,302 + SGL_RS13090 0.66 +0.9 2,408,310 - SGL_RS13090 0.66 +0.5 2,408,325 + SGL_RS13090 0.68 -0.2 2,408,325 - SGL_RS13090 0.68 -0.0 2,408,404 + SGL_RS13090 0.75 +0.8
Or see this region's nucleotide sequence