Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS18370 and SGL_RS18375 are separated by 148 nucleotides SGL_RS18375 and SGL_RS18380 are separated by 104 nucleotides SGL_RS18380 and SGL_RS18385 are separated by 135 nucleotides
SGL_RS18370: SGL_RS18370 - cyclic nucleotide-binding domain-containing protein, at 3,569,134 to 3,569,523
_RS18370
SGL_RS18375: SGL_RS18375 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 3,569,672 to 3,570,319
_RS18375
SGL_RS18380: SGL_RS18380 - GTP-binding protein, at 3,570,424 to 3,571,575
_RS18380
SGL_RS18385: SGL_RS18385 - ABC transporter permease, at 3,571,711 to 3,572,403
_RS18385
Position (kb)
3570
3571
3572 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3569.561 kb on + strand at 3570.460 kb on - strand at 3570.789 kb on + strand, within SGL_RS18380 at 3571.002 kb on + strand, within SGL_RS18380 at 3571.239 kb on - strand, within SGL_RS18380 at 3571.261 kb on + strand, within SGL_RS18380 at 3571.494 kb on - strand at 3571.523 kb on - strand at 3571.558 kb on + strand at 3571.683 kb on - strand at 3571.695 kb on - strand at 3571.771 kb on + strand at 3571.771 kb on + strand at 3571.779 kb on - strand at 3571.824 kb on + strand, within SGL_RS18385 at 3571.832 kb on - strand, within SGL_RS18385 at 3571.842 kb on - strand, within SGL_RS18385 at 3571.965 kb on + strand, within SGL_RS18385 at 3572.187 kb on + strand, within SGL_RS18385 at 3572.205 kb on - strand, within SGL_RS18385 at 3572.210 kb on - strand, within SGL_RS18385 at 3572.234 kb on + strand, within SGL_RS18385 at 3572.286 kb on + strand, within SGL_RS18385 at 3572.286 kb on - strand, within SGL_RS18385 at 3572.314 kb on - strand, within SGL_RS18385 at 3572.475 kb on - strand at 3572.501 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 3,569,561 + -0.1 3,570,460 - -2.2 3,570,789 + SGL_RS18380 0.32 -0.8 3,571,002 + SGL_RS18380 0.50 -0.6 3,571,239 - SGL_RS18380 0.71 +1.8 3,571,261 + SGL_RS18380 0.73 -0.7 3,571,494 - -1.2 3,571,523 - -0.5 3,571,558 + -0.3 3,571,683 - -0.0 3,571,695 - -0.3 3,571,771 + -0.2 3,571,771 + +0.2 3,571,779 - +0.8 3,571,824 + SGL_RS18385 0.16 -0.4 3,571,832 - SGL_RS18385 0.17 -0.8 3,571,842 - SGL_RS18385 0.19 +0.6 3,571,965 + SGL_RS18385 0.37 +0.1 3,572,187 + SGL_RS18385 0.69 -0.1 3,572,205 - SGL_RS18385 0.71 -1.2 3,572,210 - SGL_RS18385 0.72 -0.3 3,572,234 + SGL_RS18385 0.75 -1.2 3,572,286 + SGL_RS18385 0.83 +0.4 3,572,286 - SGL_RS18385 0.83 +0.7 3,572,314 - SGL_RS18385 0.87 +1.1 3,572,475 - -0.6 3,572,501 - -0.9
Or see this region's nucleotide sequence