Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14860 and SGL_RS14865 are separated by 157 nucleotides SGL_RS14865 and SGL_RS14870 are separated by 5 nucleotides SGL_RS14870 and SGL_RS14875 are separated by 49 nucleotides
SGL_RS14860: SGL_RS14860 - 8-amino-7-oxononanoate synthase, at 2,791,820 to 2,793,001
_RS14860
SGL_RS14865: SGL_RS14865 - type I methionyl aminopeptidase, at 2,793,159 to 2,793,920
_RS14865
SGL_RS14870: SGL_RS14870 - thiol-disulfide oxidoreductase DCC family protein, at 2,793,926 to 2,794,381
_RS14870
SGL_RS14875: SGL_RS14875 - NUDIX hydrolase, at 2,794,431 to 2,794,880
_RS14875
Position (kb)
2793
2794 Strain fitness (log2 ratio)
-1
0
1
2 at 2792.228 kb on - strand, within SGL_RS14860 at 2792.501 kb on - strand, within SGL_RS14860 at 2792.542 kb on + strand, within SGL_RS14860 at 2792.555 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.560 kb on - strand, within SGL_RS14860 at 2792.643 kb on + strand, within SGL_RS14860 at 2792.806 kb on + strand, within SGL_RS14860 at 2792.932 kb on + strand at 2792.946 kb on + strand at 2793.026 kb on - strand at 2793.065 kb on + strand at 2793.177 kb on - strand at 2793.231 kb on + strand at 2793.481 kb on - strand, within SGL_RS14865 at 2793.850 kb on - strand at 2794.007 kb on + strand, within SGL_RS14870 at 2794.061 kb on + strand, within SGL_RS14870 at 2794.069 kb on - strand, within SGL_RS14870 at 2794.069 kb on - strand, within SGL_RS14870 at 2794.121 kb on + strand, within SGL_RS14870 at 2794.415 kb on + strand at 2794.468 kb on - strand at 2794.532 kb on + strand, within SGL_RS14875 at 2794.581 kb on - strand, within SGL_RS14875 at 2794.640 kb on + strand, within SGL_RS14875 at 2794.789 kb on + strand, within SGL_RS14875
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,792,228 - SGL_RS14860 0.35 +1.8 2,792,501 - SGL_RS14860 0.58 +1.3 2,792,542 + SGL_RS14860 0.61 +0.0 2,792,555 - SGL_RS14860 0.62 +2.3 2,792,560 - SGL_RS14860 0.63 +1.3 2,792,560 - SGL_RS14860 0.63 +0.3 2,792,560 - SGL_RS14860 0.63 +0.8 2,792,643 + SGL_RS14860 0.70 +1.3 2,792,806 + SGL_RS14860 0.83 +2.3 2,792,932 + +0.8 2,792,946 + +0.9 2,793,026 - -0.7 2,793,065 + +0.5 2,793,177 - -0.9 2,793,231 + -0.8 2,793,481 - SGL_RS14865 0.42 -0.7 2,793,850 - +0.3 2,794,007 + SGL_RS14870 0.18 -0.6 2,794,061 + SGL_RS14870 0.30 -0.1 2,794,069 - SGL_RS14870 0.31 -0.8 2,794,069 - SGL_RS14870 0.31 -0.1 2,794,121 + SGL_RS14870 0.43 +0.8 2,794,415 + +0.7 2,794,468 - +0.6 2,794,532 + SGL_RS14875 0.22 -0.2 2,794,581 - SGL_RS14875 0.33 -0.8 2,794,640 + SGL_RS14875 0.46 -0.6 2,794,789 + SGL_RS14875 0.80 -0.1
Or see this region's nucleotide sequence