Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14675 and SGL_RS14680 are separated by 167 nucleotides SGL_RS14680 and SGL_RS14685 are separated by 126 nucleotides SGL_RS14685 and SGL_RS14690 are separated by 269 nucleotides
SGL_RS14675: SGL_RS14675 - peptidylprolyl isomerase, at 2,744,987 to 2,745,766
_RS14675
SGL_RS14680: SGL_RS14680 - ferredoxin-thioredoxin reductase variable chain, at 2,745,934 to 2,746,161
_RS14680
SGL_RS14685: SGL_RS14685 - hypothetical protein, at 2,746,288 to 2,747,334
_RS14685
SGL_RS14690: SGL_RS14690 - HAMP domain-containing sensor histidine kinase, at 2,747,604 to 2,748,857
_RS14690
Position (kb)
2746
2747
2748 Strain fitness (log2 ratio)
-1
0
1 at 2745.461 kb on - strand, within SGL_RS14675 at 2745.653 kb on + strand, within SGL_RS14675 at 2745.755 kb on + strand at 2746.139 kb on + strand at 2746.293 kb on - strand at 2746.396 kb on - strand, within SGL_RS14685 at 2746.600 kb on + strand, within SGL_RS14685 at 2746.799 kb on - strand, within SGL_RS14685 at 2746.833 kb on + strand, within SGL_RS14685 at 2746.961 kb on + strand, within SGL_RS14685 at 2747.336 kb on - strand at 2747.618 kb on + strand at 2747.653 kb on + strand at 2747.732 kb on - strand, within SGL_RS14690 at 2747.786 kb on - strand, within SGL_RS14690 at 2747.804 kb on + strand, within SGL_RS14690 at 2747.812 kb on - strand, within SGL_RS14690 at 2747.869 kb on - strand, within SGL_RS14690 at 2747.886 kb on + strand, within SGL_RS14690 at 2748.238 kb on + strand, within SGL_RS14690
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,745,461 - SGL_RS14675 0.61 -0.5 2,745,653 + SGL_RS14675 0.85 +0.3 2,745,755 + -0.4 2,746,139 + -0.3 2,746,293 - -0.0 2,746,396 - SGL_RS14685 0.10 -1.7 2,746,600 + SGL_RS14685 0.30 -0.2 2,746,799 - SGL_RS14685 0.49 -0.8 2,746,833 + SGL_RS14685 0.52 -0.4 2,746,961 + SGL_RS14685 0.64 -0.1 2,747,336 - -0.2 2,747,618 + -1.2 2,747,653 + -0.1 2,747,732 - SGL_RS14690 0.10 +0.4 2,747,786 - SGL_RS14690 0.15 -1.3 2,747,804 + SGL_RS14690 0.16 -0.4 2,747,812 - SGL_RS14690 0.17 +0.5 2,747,869 - SGL_RS14690 0.21 -0.8 2,747,886 + SGL_RS14690 0.22 -0.6 2,748,238 + SGL_RS14690 0.51 -0.1
Or see this region's nucleotide sequence