Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14070 and SGL_RS14075 are separated by 102 nucleotides SGL_RS14075 and SGL_RS14080 are separated by 177 nucleotides SGL_RS14080 and SGL_RS14085 are separated by 76 nucleotides SGL_RS14085 and SGL_RS14090 are separated by 135 nucleotides
SGL_RS14070: SGL_RS14070 - class I SAM-dependent methyltransferase, at 2,616,624 to 2,617,382
_RS14070
SGL_RS14075: SGL_RS14075 - CAAD domain-containing protein, at 2,617,485 to 2,617,889
_RS14075
SGL_RS14080: SGL_RS14080 - DICT sensory domain-containing protein, at 2,618,067 to 2,620,094
_RS14080
SGL_RS14085: SGL_RS14085 - PAS domain-containing protein, at 2,620,171 to 2,620,662
_RS14085
SGL_RS14090: SGL_RS14090 - murein biosynthesis integral membrane protein MurJ, at 2,620,798 to 2,622,399
_RS14090
Position (kb)
2618
2619
2620
2621 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2617.125 kb on - strand, within SGL_RS14070 at 2617.184 kb on - strand, within SGL_RS14070 at 2617.213 kb on + strand, within SGL_RS14070 at 2617.307 kb on + strand at 2617.340 kb on - strand at 2618.032 kb on - strand at 2618.154 kb on + strand at 2618.154 kb on + strand at 2618.154 kb on + strand at 2618.162 kb on - strand at 2618.167 kb on - strand at 2618.353 kb on + strand, within SGL_RS14080 at 2618.451 kb on + strand, within SGL_RS14080 at 2618.622 kb on + strand, within SGL_RS14080 at 2618.694 kb on + strand, within SGL_RS14080 at 2618.696 kb on + strand, within SGL_RS14080 at 2618.704 kb on - strand, within SGL_RS14080 at 2618.713 kb on - strand, within SGL_RS14080 at 2618.800 kb on + strand, within SGL_RS14080 at 2618.929 kb on - strand, within SGL_RS14080 at 2618.959 kb on - strand, within SGL_RS14080 at 2618.994 kb on - strand, within SGL_RS14080 at 2619.098 kb on - strand, within SGL_RS14080 at 2619.177 kb on + strand, within SGL_RS14080 at 2619.211 kb on - strand, within SGL_RS14080 at 2619.246 kb on + strand, within SGL_RS14080 at 2619.247 kb on - strand, within SGL_RS14080 at 2619.299 kb on - strand, within SGL_RS14080 at 2619.299 kb on - strand, within SGL_RS14080 at 2619.316 kb on - strand, within SGL_RS14080 at 2619.316 kb on - strand, within SGL_RS14080 at 2619.368 kb on - strand, within SGL_RS14080 at 2619.395 kb on + strand, within SGL_RS14080 at 2619.409 kb on + strand, within SGL_RS14080 at 2619.443 kb on - strand, within SGL_RS14080 at 2619.552 kb on - strand, within SGL_RS14080 at 2619.605 kb on - strand, within SGL_RS14080 at 2619.773 kb on + strand, within SGL_RS14080 at 2619.781 kb on - strand, within SGL_RS14080 at 2619.791 kb on - strand, within SGL_RS14080 at 2619.805 kb on - strand, within SGL_RS14080 at 2619.874 kb on + strand, within SGL_RS14080 at 2620.166 kb on + strand at 2620.240 kb on + strand, within SGL_RS14085 at 2620.385 kb on - strand, within SGL_RS14085 at 2620.387 kb on + strand, within SGL_RS14085 at 2620.494 kb on - strand, within SGL_RS14085 at 2620.509 kb on + strand, within SGL_RS14085 at 2620.522 kb on - strand, within SGL_RS14085 at 2620.580 kb on + strand, within SGL_RS14085 at 2620.612 kb on + strand, within SGL_RS14085 at 2620.658 kb on + strand at 2620.689 kb on - strand at 2620.736 kb on + strand at 2620.744 kb on - strand at 2620.744 kb on - strand at 2620.744 kb on - strand at 2620.744 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,617,125 - SGL_RS14070 0.66 -0.3 2,617,184 - SGL_RS14070 0.74 -0.3 2,617,213 + SGL_RS14070 0.78 +0.1 2,617,307 + +1.0 2,617,340 - -1.4 2,618,032 - -1.4 2,618,154 + -1.6 2,618,154 + -1.7 2,618,154 + -0.8 2,618,162 - -2.1 2,618,167 - -2.2 2,618,353 + SGL_RS14080 0.14 +0.1 2,618,451 + SGL_RS14080 0.19 -1.4 2,618,622 + SGL_RS14080 0.27 +0.5 2,618,694 + SGL_RS14080 0.31 -3.2 2,618,696 + SGL_RS14080 0.31 -1.2 2,618,704 - SGL_RS14080 0.31 -1.0 2,618,713 - SGL_RS14080 0.32 -1.3 2,618,800 + SGL_RS14080 0.36 -1.9 2,618,929 - SGL_RS14080 0.43 -1.0 2,618,959 - SGL_RS14080 0.44 -2.0 2,618,994 - SGL_RS14080 0.46 -0.8 2,619,098 - SGL_RS14080 0.51 -1.4 2,619,177 + SGL_RS14080 0.55 -1.9 2,619,211 - SGL_RS14080 0.56 -2.1 2,619,246 + SGL_RS14080 0.58 -0.9 2,619,247 - SGL_RS14080 0.58 -1.9 2,619,299 - SGL_RS14080 0.61 +0.0 2,619,299 - SGL_RS14080 0.61 -1.5 2,619,316 - SGL_RS14080 0.62 -1.9 2,619,316 - SGL_RS14080 0.62 -2.1 2,619,368 - SGL_RS14080 0.64 -1.5 2,619,395 + SGL_RS14080 0.65 -2.3 2,619,409 + SGL_RS14080 0.66 -1.4 2,619,443 - SGL_RS14080 0.68 -1.7 2,619,552 - SGL_RS14080 0.73 -1.5 2,619,605 - SGL_RS14080 0.76 -1.8 2,619,773 + SGL_RS14080 0.84 -2.3 2,619,781 - SGL_RS14080 0.85 -1.4 2,619,791 - SGL_RS14080 0.85 -0.8 2,619,805 - SGL_RS14080 0.86 -1.2 2,619,874 + SGL_RS14080 0.89 -2.8 2,620,166 + -0.2 2,620,240 + SGL_RS14085 0.14 -0.2 2,620,385 - SGL_RS14085 0.43 +0.3 2,620,387 + SGL_RS14085 0.44 +0.2 2,620,494 - SGL_RS14085 0.66 -0.3 2,620,509 + SGL_RS14085 0.69 +0.3 2,620,522 - SGL_RS14085 0.71 -0.1 2,620,580 + SGL_RS14085 0.83 -0.0 2,620,612 + SGL_RS14085 0.90 +0.1 2,620,658 + +0.1 2,620,689 - -0.2 2,620,736 + -0.9 2,620,744 - -0.1 2,620,744 - -0.6 2,620,744 - +1.0 2,620,744 - -0.6
Or see this region's nucleotide sequence