Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13790 and SGL_RS13795 are separated by 193 nucleotides SGL_RS13795 and SGL_RS13800 are separated by 62 nucleotides SGL_RS13800 and SGL_RS13805 are separated by 93 nucleotides
SGL_RS13790: SGL_RS13790 - citrate synthase, at 2,560,112 to 2,561,305
_RS13790
SGL_RS13795: SGL_RS13795 - phosphohistidine phosphatase SixA, at 2,561,499 to 2,561,993
_RS13795
SGL_RS13800: SGL_RS13800 - alpha/beta hydrolase, at 2,562,056 to 2,562,859
_RS13800
SGL_RS13805: SGL_RS13805 - saccharopine dehydrogenase family protein, at 2,562,953 to 2,564,149
_RS13805
Position (kb)
2561
2562 Strain fitness (log2 ratio)
-1
0
1 at 2561.378 kb on + strand at 2561.435 kb on + strand at 2561.435 kb on + strand at 2561.443 kb on - strand at 2561.447 kb on + strand at 2561.447 kb on + strand at 2561.455 kb on - strand at 2561.505 kb on - strand at 2561.511 kb on + strand at 2561.896 kb on + strand, within SGL_RS13795 at 2561.944 kb on + strand at 2561.954 kb on + strand at 2562.258 kb on - strand, within SGL_RS13800 at 2562.345 kb on + strand, within SGL_RS13800 at 2562.495 kb on + strand, within SGL_RS13800 at 2562.796 kb on + strand at 2562.828 kb on - strand at 2562.867 kb on + strand at 2562.877 kb on + strand at 2562.877 kb on + strand at 2562.885 kb on - strand at 2562.926 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,561,378 + +1.3 2,561,435 + +0.4 2,561,435 + -0.1 2,561,443 - -0.5 2,561,447 + -0.1 2,561,447 + -0.5 2,561,455 - -0.2 2,561,505 - -0.2 2,561,511 + -0.2 2,561,896 + SGL_RS13795 0.80 -1.5 2,561,944 + +0.3 2,561,954 + -0.3 2,562,258 - SGL_RS13800 0.25 -0.1 2,562,345 + SGL_RS13800 0.36 -0.4 2,562,495 + SGL_RS13800 0.55 -0.1 2,562,796 + -0.3 2,562,828 - +0.2 2,562,867 + -0.6 2,562,877 + -0.1 2,562,877 + +0.9 2,562,885 - -0.3 2,562,926 + +0.7
Or see this region's nucleotide sequence