Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12895 and SGL_RS12900 are separated by 100 nucleotides SGL_RS12900 and SGL_RS12905 are separated by 171 nucleotides
SGL_RS12895: SGL_RS12895 - efflux RND transporter permease subunit, at 2,365,930 to 2,369,181
_RS12895
SGL_RS12900: SGL_RS12900 - NAD-dependent succinate-semialdehyde dehydrogenase, at 2,369,282 to 2,370,646
_RS12900
SGL_RS12905: SGL_RS12905 - PAS domain-containing sensor histidine kinase, at 2,370,818 to 2,372,110
_RS12905
Position (kb)
2369
2370
2371 Strain fitness (log2 ratio)
-2
-1
0
1 at 2368.449 kb on + strand, within SGL_RS12895 at 2368.480 kb on + strand, within SGL_RS12895 at 2368.570 kb on + strand, within SGL_RS12895 at 2368.664 kb on + strand, within SGL_RS12895 at 2368.738 kb on + strand, within SGL_RS12895 at 2369.041 kb on - strand at 2369.058 kb on + strand at 2369.066 kb on - strand at 2369.272 kb on + strand at 2369.273 kb on + strand at 2369.281 kb on - strand at 2369.281 kb on - strand at 2369.295 kb on + strand at 2369.345 kb on - strand at 2369.348 kb on + strand at 2369.488 kb on - strand, within SGL_RS12900 at 2369.618 kb on - strand, within SGL_RS12900 at 2369.698 kb on - strand, within SGL_RS12900 at 2369.735 kb on - strand, within SGL_RS12900 at 2369.748 kb on + strand, within SGL_RS12900 at 2369.828 kb on + strand, within SGL_RS12900 at 2369.918 kb on + strand, within SGL_RS12900 at 2370.013 kb on - strand, within SGL_RS12900 at 2370.130 kb on - strand, within SGL_RS12900 at 2370.193 kb on + strand, within SGL_RS12900 at 2370.337 kb on - strand, within SGL_RS12900 at 2370.611 kb on - strand at 2370.641 kb on + strand at 2370.681 kb on - strand at 2370.681 kb on - strand at 2370.952 kb on - strand, within SGL_RS12905 at 2371.177 kb on + strand, within SGL_RS12905 at 2371.278 kb on - strand, within SGL_RS12905 at 2371.420 kb on - strand, within SGL_RS12905 at 2371.524 kb on - strand, within SGL_RS12905
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,368,449 + SGL_RS12895 0.77 -0.5 2,368,480 + SGL_RS12895 0.78 +0.7 2,368,570 + SGL_RS12895 0.81 +0.1 2,368,664 + SGL_RS12895 0.84 -0.9 2,368,738 + SGL_RS12895 0.86 -0.4 2,369,041 - +0.3 2,369,058 + -0.2 2,369,066 - -0.1 2,369,272 + -0.8 2,369,273 + -0.3 2,369,281 - -0.7 2,369,281 - -1.0 2,369,295 + -1.1 2,369,345 - -1.0 2,369,348 + -1.8 2,369,488 - SGL_RS12900 0.15 -1.5 2,369,618 - SGL_RS12900 0.25 -0.6 2,369,698 - SGL_RS12900 0.30 -1.0 2,369,735 - SGL_RS12900 0.33 -0.9 2,369,748 + SGL_RS12900 0.34 -0.4 2,369,828 + SGL_RS12900 0.40 -0.5 2,369,918 + SGL_RS12900 0.47 -0.5 2,370,013 - SGL_RS12900 0.54 -1.8 2,370,130 - SGL_RS12900 0.62 -1.2 2,370,193 + SGL_RS12900 0.67 -0.2 2,370,337 - SGL_RS12900 0.77 -0.2 2,370,611 - -0.7 2,370,641 + +0.6 2,370,681 - -1.1 2,370,681 - -0.2 2,370,952 - SGL_RS12905 0.10 -1.1 2,371,177 + SGL_RS12905 0.28 +0.7 2,371,278 - SGL_RS12905 0.36 -0.2 2,371,420 - SGL_RS12905 0.47 -0.1 2,371,524 - SGL_RS12905 0.55 -0.9
Or see this region's nucleotide sequence