Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12055 and SGL_RS12060 are separated by 17 nucleotides SGL_RS12060 and SGL_RS12065 are separated by 100 nucleotides SGL_RS12065 and SGL_RS19235 are separated by 78 nucleotides SGL_RS19235 and SGL_RS12070 are separated by 60 nucleotides SGL_RS12070 and SGL_RS12075 are separated by 153 nucleotides
SGL_RS12055: SGL_RS12055 - DUF6439 family protein, at 2,154,205 to 2,154,498
_RS12055
SGL_RS12060: SGL_RS12060 - phosphoribosylanthranilate isomerase, at 2,154,516 to 2,155,172
_RS12060
SGL_RS12065: SGL_RS12065 - DMT family transporter, at 2,155,273 to 2,156,265
_RS12065
SGL_RS19235: SGL_RS19235 - IS1 family transposase, at 2,156,344 to 2,156,436
_RS19235
SGL_RS12070: SGL_RS12070 - ABA4-like family protein, at 2,156,497 to 2,156,961
_RS12070
SGL_RS12075: SGL_RS12075 - DUF2442 domain-containing protein, at 2,157,115 to 2,157,303
_RS12075
Position (kb)
2155
2156
2157 Strain fitness (log2 ratio)
-2
-1
0
1 at 2154.362 kb on - strand, within SGL_RS12055 at 2154.403 kb on + strand, within SGL_RS12055 at 2154.411 kb on - strand, within SGL_RS12055 at 2154.411 kb on - strand, within SGL_RS12055 at 2154.464 kb on + strand, within SGL_RS12055 at 2154.550 kb on + strand at 2155.325 kb on + strand at 2155.333 kb on - strand at 2155.380 kb on + strand, within SGL_RS12065 at 2155.405 kb on - strand, within SGL_RS12065 at 2155.425 kb on + strand, within SGL_RS12065 at 2155.527 kb on - strand, within SGL_RS12065 at 2155.557 kb on - strand, within SGL_RS12065 at 2155.580 kb on - strand, within SGL_RS12065 at 2155.711 kb on - strand, within SGL_RS12065 at 2155.735 kb on + strand, within SGL_RS12065 at 2155.830 kb on + strand, within SGL_RS12065 at 2155.965 kb on - strand, within SGL_RS12065 at 2155.965 kb on - strand, within SGL_RS12065 at 2155.965 kb on - strand, within SGL_RS12065 at 2155.965 kb on - strand, within SGL_RS12065 at 2155.978 kb on - strand, within SGL_RS12065 at 2155.986 kb on - strand, within SGL_RS12065 at 2155.992 kb on - strand, within SGL_RS12065 at 2156.077 kb on + strand, within SGL_RS12065 at 2156.090 kb on + strand, within SGL_RS12065 at 2156.125 kb on - strand, within SGL_RS12065 at 2156.287 kb on + strand at 2156.561 kb on - strand, within SGL_RS12070 at 2156.589 kb on + strand, within SGL_RS12070 at 2156.760 kb on + strand, within SGL_RS12070 at 2156.768 kb on - strand, within SGL_RS12070 at 2156.768 kb on - strand, within SGL_RS12070 at 2156.770 kb on + strand, within SGL_RS12070 at 2156.927 kb on + strand at 2156.928 kb on + strand at 2157.045 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 2,154,362 - SGL_RS12055 0.53 +0.1 2,154,403 + SGL_RS12055 0.67 -0.2 2,154,411 - SGL_RS12055 0.70 -0.2 2,154,411 - SGL_RS12055 0.70 +0.8 2,154,464 + SGL_RS12055 0.88 +0.4 2,154,550 + +0.6 2,155,325 + -1.4 2,155,333 - -0.4 2,155,380 + SGL_RS12065 0.11 -2.3 2,155,405 - SGL_RS12065 0.13 -1.3 2,155,425 + SGL_RS12065 0.15 -1.3 2,155,527 - SGL_RS12065 0.26 -1.2 2,155,557 - SGL_RS12065 0.29 -2.0 2,155,580 - SGL_RS12065 0.31 -1.7 2,155,711 - SGL_RS12065 0.44 -1.5 2,155,735 + SGL_RS12065 0.47 -1.4 2,155,830 + SGL_RS12065 0.56 -1.6 2,155,965 - SGL_RS12065 0.70 -1.9 2,155,965 - SGL_RS12065 0.70 -2.3 2,155,965 - SGL_RS12065 0.70 -1.0 2,155,965 - SGL_RS12065 0.70 -0.2 2,155,978 - SGL_RS12065 0.71 +0.7 2,155,986 - SGL_RS12065 0.72 -2.4 2,155,992 - SGL_RS12065 0.72 -1.0 2,156,077 + SGL_RS12065 0.81 -1.8 2,156,090 + SGL_RS12065 0.82 -1.6 2,156,125 - SGL_RS12065 0.86 -1.1 2,156,287 + -1.4 2,156,561 - SGL_RS12070 0.14 +0.0 2,156,589 + SGL_RS12070 0.20 -0.2 2,156,760 + SGL_RS12070 0.57 -0.5 2,156,768 - SGL_RS12070 0.58 +0.1 2,156,768 - SGL_RS12070 0.58 +0.6 2,156,770 + SGL_RS12070 0.59 +0.6 2,156,927 + -1.5 2,156,928 + -0.1 2,157,045 + +1.3
Or see this region's nucleotide sequence