Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS08255 and SGL_RS08260 are separated by 100 nucleotides SGL_RS08260 and SGL_RS08265 overlap by 8 nucleotides SGL_RS08265 and SGL_RS08270 overlap by 22 nucleotides
SGL_RS08255: SGL_RS08255 - ABC-F family ATP-binding cassette domain-containing protein, at 1,360,434 to 1,362,158
_RS08255
SGL_RS08260: SGL_RS08260 - L-histidine N(alpha)-methyltransferase, at 1,362,259 to 1,363,272
_RS08260
SGL_RS08265: SGL_RS08265 - hypothetical protein, at 1,363,265 to 1,363,858
_RS08265
SGL_RS08270: SGL_RS08270 - orotidine-5'-phosphate decarboxylase, at 1,363,837 to 1,364,532
_RS08270
Position (kb)
1362
1363
1364 Strain fitness (log2 ratio)
-2
-1
0
1 at 1361.279 kb on + strand, within SGL_RS08255 at 1361.453 kb on + strand, within SGL_RS08255 at 1362.003 kb on + strand at 1362.009 kb on - strand at 1362.134 kb on - strand at 1362.183 kb on + strand at 1362.215 kb on + strand at 1362.223 kb on - strand at 1362.223 kb on - strand at 1362.266 kb on + strand at 1362.316 kb on + strand at 1362.515 kb on - strand, within SGL_RS08260 at 1362.658 kb on + strand, within SGL_RS08260 at 1363.202 kb on + strand at 1363.227 kb on - strand at 1363.296 kb on + strand at 1363.338 kb on - strand, within SGL_RS08265 at 1363.415 kb on + strand, within SGL_RS08265 at 1363.418 kb on - strand, within SGL_RS08265 at 1363.479 kb on + strand, within SGL_RS08265 at 1363.493 kb on - strand, within SGL_RS08265 at 1363.680 kb on - strand, within SGL_RS08265 at 1363.697 kb on + strand, within SGL_RS08265 at 1363.759 kb on - strand, within SGL_RS08265
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 1,361,279 + SGL_RS08255 0.49 -0.3 1,361,453 + SGL_RS08255 0.59 -2.2 1,362,003 + +0.7 1,362,009 - -0.3 1,362,134 - +0.3 1,362,183 + +0.2 1,362,215 + -0.2 1,362,223 - +0.1 1,362,223 - -0.1 1,362,266 + -0.6 1,362,316 + -0.3 1,362,515 - SGL_RS08260 0.25 -0.9 1,362,658 + SGL_RS08260 0.39 -0.7 1,363,202 + -0.0 1,363,227 - -0.6 1,363,296 + +0.0 1,363,338 - SGL_RS08265 0.12 -0.4 1,363,415 + SGL_RS08265 0.25 -1.5 1,363,418 - SGL_RS08265 0.26 -0.9 1,363,479 + SGL_RS08265 0.36 +0.1 1,363,493 - SGL_RS08265 0.38 -0.6 1,363,680 - SGL_RS08265 0.70 -0.4 1,363,697 + SGL_RS08265 0.73 -0.1 1,363,759 - SGL_RS08265 0.83 -1.5
Or see this region's nucleotide sequence