Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07525 and SGL_RS07530 are separated by 49 nucleotides SGL_RS07530 and SGL_RS07535 are separated by 129 nucleotides SGL_RS07535 and SGL_RS07540 overlap by 13 nucleotides
SGL_RS07525: SGL_RS07525 - CDGSH iron-sulfur domain-containing protein, at 1,215,167 to 1,215,403
_RS07525
SGL_RS07530: SGL_RS07530 - pirin family protein, at 1,215,453 to 1,216,151
_RS07530
SGL_RS07535: SGL_RS07535 - LysR family transcriptional regulator, at 1,216,281 to 1,217,168
_RS07535
SGL_RS07540: SGL_RS07540 - endonuclease MutS2, at 1,217,156 to 1,219,624
_RS07540
Position (kb)
1216
1217
1218 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1215.336 kb on + strand, within SGL_RS07525 at 1215.344 kb on - strand, within SGL_RS07525 at 1215.450 kb on + strand at 1215.451 kb on + strand at 1215.458 kb on - strand at 1215.587 kb on + strand, within SGL_RS07530 at 1215.634 kb on - strand, within SGL_RS07530 at 1215.639 kb on + strand, within SGL_RS07530 at 1215.725 kb on - strand, within SGL_RS07530 at 1215.785 kb on + strand, within SGL_RS07530 at 1215.793 kb on - strand, within SGL_RS07530 at 1215.872 kb on - strand, within SGL_RS07530 at 1215.876 kb on + strand, within SGL_RS07530 at 1215.879 kb on - strand, within SGL_RS07530 at 1216.119 kb on + strand at 1216.144 kb on - strand at 1216.362 kb on - strand at 1216.553 kb on - strand, within SGL_RS07535 at 1216.567 kb on + strand, within SGL_RS07535 at 1216.589 kb on - strand, within SGL_RS07535 at 1216.627 kb on - strand, within SGL_RS07535 at 1216.783 kb on - strand, within SGL_RS07535 at 1216.795 kb on + strand, within SGL_RS07535 at 1216.964 kb on - strand, within SGL_RS07535 at 1216.964 kb on - strand, within SGL_RS07535 at 1216.979 kb on + strand, within SGL_RS07535 at 1217.057 kb on + strand, within SGL_RS07535 at 1217.074 kb on + strand, within SGL_RS07535 at 1217.463 kb on + strand, within SGL_RS07540 at 1217.463 kb on + strand, within SGL_RS07540 at 1217.574 kb on + strand, within SGL_RS07540 at 1217.582 kb on - strand, within SGL_RS07540 at 1217.642 kb on - strand, within SGL_RS07540 at 1217.728 kb on - strand, within SGL_RS07540 at 1217.736 kb on + strand, within SGL_RS07540 at 1217.744 kb on + strand, within SGL_RS07540 at 1217.949 kb on + strand, within SGL_RS07540 at 1217.957 kb on - strand, within SGL_RS07540 at 1217.972 kb on + strand, within SGL_RS07540 at 1218.011 kb on - strand, within SGL_RS07540 at 1218.044 kb on - strand, within SGL_RS07540 at 1218.079 kb on - strand, within SGL_RS07540 at 1218.103 kb on + strand, within SGL_RS07540
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 1,215,336 + SGL_RS07525 0.71 -0.1 1,215,344 - SGL_RS07525 0.75 -0.4 1,215,450 + -0.6 1,215,451 + +0.2 1,215,458 - +0.6 1,215,587 + SGL_RS07530 0.19 +0.6 1,215,634 - SGL_RS07530 0.26 +0.4 1,215,639 + SGL_RS07530 0.27 -0.4 1,215,725 - SGL_RS07530 0.39 +0.6 1,215,785 + SGL_RS07530 0.47 -0.4 1,215,793 - SGL_RS07530 0.49 +0.1 1,215,872 - SGL_RS07530 0.60 -0.8 1,215,876 + SGL_RS07530 0.61 +0.1 1,215,879 - SGL_RS07530 0.61 -0.5 1,216,119 + -0.3 1,216,144 - -0.2 1,216,362 - -1.1 1,216,553 - SGL_RS07535 0.31 -0.2 1,216,567 + SGL_RS07535 0.32 -2.0 1,216,589 - SGL_RS07535 0.35 -1.2 1,216,627 - SGL_RS07535 0.39 -0.4 1,216,783 - SGL_RS07535 0.57 -1.0 1,216,795 + SGL_RS07535 0.58 +0.4 1,216,964 - SGL_RS07535 0.77 -0.8 1,216,964 - SGL_RS07535 0.77 -0.6 1,216,979 + SGL_RS07535 0.79 -1.3 1,217,057 + SGL_RS07535 0.87 +0.0 1,217,074 + SGL_RS07535 0.89 -1.2 1,217,463 + SGL_RS07540 0.12 +0.3 1,217,463 + SGL_RS07540 0.12 +0.1 1,217,574 + SGL_RS07540 0.17 +2.2 1,217,582 - SGL_RS07540 0.17 +0.2 1,217,642 - SGL_RS07540 0.20 -0.2 1,217,728 - SGL_RS07540 0.23 +0.9 1,217,736 + SGL_RS07540 0.23 -0.1 1,217,744 + SGL_RS07540 0.24 +0.2 1,217,949 + SGL_RS07540 0.32 +0.7 1,217,957 - SGL_RS07540 0.32 -0.0 1,217,972 + SGL_RS07540 0.33 -0.2 1,218,011 - SGL_RS07540 0.35 -0.8 1,218,044 - SGL_RS07540 0.36 +0.8 1,218,079 - SGL_RS07540 0.37 -0.4 1,218,103 + SGL_RS07540 0.38 -0.3
Or see this region's nucleotide sequence