Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07165 and SGL_RS07170 are separated by 97 nucleotides SGL_RS07170 and SGL_RS07175 are separated by 93 nucleotides SGL_RS07175 and SGL_RS07180 are separated by 22 nucleotides
SGL_RS07165: SGL_RS07165 - M23 family metallopeptidase, at 1,137,216 to 1,138,112
_RS07165
SGL_RS07170: SGL_RS07170 - glycine cleavage system protein GcvH, at 1,138,210 to 1,138,608
_RS07170
SGL_RS07175: SGL_RS07175 - NFACT family protein, at 1,138,702 to 1,140,474
_RS07175
SGL_RS07180: SGL_RS07180 - carboxynorspermidine decarboxylase, at 1,140,497 to 1,141,645
_RS07180
Position (kb)
1138
1139
1140
1141 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 1138.309 kb on + strand, within SGL_RS07170 at 1138.385 kb on - strand, within SGL_RS07170 at 1138.414 kb on + strand, within SGL_RS07170 at 1138.578 kb on + strand at 1138.589 kb on + strand at 1138.623 kb on + strand at 1138.623 kb on + strand at 1138.723 kb on + strand at 1138.746 kb on + strand at 1138.804 kb on + strand at 1138.877 kb on + strand at 1138.947 kb on + strand, within SGL_RS07175 at 1138.947 kb on + strand, within SGL_RS07175 at 1138.955 kb on - strand, within SGL_RS07175 at 1139.046 kb on - strand, within SGL_RS07175 at 1139.138 kb on - strand, within SGL_RS07175 at 1139.192 kb on - strand, within SGL_RS07175 at 1139.317 kb on - strand, within SGL_RS07175 at 1139.477 kb on - strand, within SGL_RS07175 at 1139.508 kb on + strand, within SGL_RS07175 at 1139.689 kb on - strand, within SGL_RS07175 at 1139.735 kb on - strand, within SGL_RS07175 at 1139.767 kb on + strand, within SGL_RS07175 at 1139.775 kb on - strand, within SGL_RS07175 at 1139.937 kb on - strand, within SGL_RS07175 at 1140.095 kb on + strand, within SGL_RS07175 at 1140.113 kb on + strand, within SGL_RS07175 at 1140.121 kb on - strand, within SGL_RS07175 at 1140.233 kb on + strand, within SGL_RS07175 at 1140.241 kb on - strand, within SGL_RS07175 at 1140.343 kb on - strand at 1140.372 kb on - strand at 1140.392 kb on - strand at 1140.460 kb on - strand at 1140.594 kb on + strand at 1140.594 kb on + strand at 1140.688 kb on - strand, within SGL_RS07180 at 1140.997 kb on - strand, within SGL_RS07180 at 1141.026 kb on + strand, within SGL_RS07180 at 1141.145 kb on - strand, within SGL_RS07180 at 1141.325 kb on - strand, within SGL_RS07180 at 1141.457 kb on + strand, within SGL_RS07180
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 1,138,309 + SGL_RS07170 0.25 +2.0 1,138,385 - SGL_RS07170 0.44 +0.6 1,138,414 + SGL_RS07170 0.51 +1.4 1,138,578 + -0.2 1,138,589 + +0.6 1,138,623 + -0.3 1,138,623 + -0.1 1,138,723 + -0.7 1,138,746 + -0.5 1,138,804 + -0.6 1,138,877 + -0.4 1,138,947 + SGL_RS07175 0.14 -0.7 1,138,947 + SGL_RS07175 0.14 -2.3 1,138,955 - SGL_RS07175 0.14 -0.1 1,139,046 - SGL_RS07175 0.19 -0.9 1,139,138 - SGL_RS07175 0.25 +0.6 1,139,192 - SGL_RS07175 0.28 -1.3 1,139,317 - SGL_RS07175 0.35 -0.6 1,139,477 - SGL_RS07175 0.44 -0.8 1,139,508 + SGL_RS07175 0.45 -0.2 1,139,689 - SGL_RS07175 0.56 -0.5 1,139,735 - SGL_RS07175 0.58 -0.6 1,139,767 + SGL_RS07175 0.60 -2.0 1,139,775 - SGL_RS07175 0.61 -0.7 1,139,937 - SGL_RS07175 0.70 -0.4 1,140,095 + SGL_RS07175 0.79 -1.1 1,140,113 + SGL_RS07175 0.80 +1.2 1,140,121 - SGL_RS07175 0.80 -1.1 1,140,233 + SGL_RS07175 0.86 -0.7 1,140,241 - SGL_RS07175 0.87 -0.9 1,140,343 - +0.2 1,140,372 - -0.4 1,140,392 - -0.1 1,140,460 - -0.8 1,140,594 + +6.5 1,140,594 + +7.2 1,140,688 - SGL_RS07180 0.17 +6.4 1,140,997 - SGL_RS07180 0.44 +6.4 1,141,026 + SGL_RS07180 0.46 +7.1 1,141,145 - SGL_RS07180 0.56 +7.2 1,141,325 - SGL_RS07180 0.72 +7.2 1,141,457 + SGL_RS07180 0.84 +6.6
Or see this region's nucleotide sequence