Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS07045 and SGL_RS07050 are separated by 120 nucleotides SGL_RS07050 and SGL_RS07055 are separated by 73 nucleotides
SGL_RS07045: SGL_RS07045 - TIGR02652 family protein, at 1,105,030 to 1,105,545
_RS07045
SGL_RS07050: SGL_RS07050 - polyphosphate:AMP phosphotransferase, at 1,105,666 to 1,107,168
_RS07050
SGL_RS07055: SGL_RS07055 - biotin synthase BioB, at 1,107,242 to 1,108,330
_RS07055
Position (kb)
1105
1106
1107
1108 Strain fitness (log2 ratio)
-2
-1
0
1 at 1104.860 kb on + strand at 1105.129 kb on - strand, within SGL_RS07045 at 1105.151 kb on + strand, within SGL_RS07045 at 1105.164 kb on + strand, within SGL_RS07045 at 1105.182 kb on - strand, within SGL_RS07045 at 1105.220 kb on + strand, within SGL_RS07045 at 1105.275 kb on + strand, within SGL_RS07045 at 1105.280 kb on + strand, within SGL_RS07045 at 1105.288 kb on - strand, within SGL_RS07045 at 1105.291 kb on + strand, within SGL_RS07045 at 1105.291 kb on + strand, within SGL_RS07045 at 1105.454 kb on - strand, within SGL_RS07045 at 1105.462 kb on + strand, within SGL_RS07045 at 1105.633 kb on - strand at 1105.661 kb on + strand at 1105.674 kb on - strand at 1105.684 kb on - strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.751 kb on + strand at 1105.759 kb on - strand at 1105.764 kb on - strand at 1106.091 kb on - strand, within SGL_RS07050 at 1106.292 kb on + strand, within SGL_RS07050 at 1106.319 kb on - strand, within SGL_RS07050 at 1106.329 kb on + strand, within SGL_RS07050 at 1106.362 kb on + strand, within SGL_RS07050 at 1106.633 kb on + strand, within SGL_RS07050 at 1106.664 kb on - strand, within SGL_RS07050 at 1106.689 kb on + strand, within SGL_RS07050 at 1106.828 kb on - strand, within SGL_RS07050 at 1106.873 kb on + strand, within SGL_RS07050 at 1106.914 kb on - strand, within SGL_RS07050 at 1106.935 kb on - strand, within SGL_RS07050 at 1106.936 kb on - strand, within SGL_RS07050 at 1106.997 kb on + strand, within SGL_RS07050 at 1107.007 kb on + strand, within SGL_RS07050 at 1107.016 kb on - strand, within SGL_RS07050 at 1107.065 kb on + strand at 1107.163 kb on + strand at 1107.171 kb on - strand at 1107.181 kb on + strand at 1107.181 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 1,104,860 + +0.1 1,105,129 - SGL_RS07045 0.19 +1.2 1,105,151 + SGL_RS07045 0.23 +0.6 1,105,164 + SGL_RS07045 0.26 -0.0 1,105,182 - SGL_RS07045 0.29 +0.1 1,105,220 + SGL_RS07045 0.37 -0.1 1,105,275 + SGL_RS07045 0.47 -0.4 1,105,280 + SGL_RS07045 0.48 +1.2 1,105,288 - SGL_RS07045 0.50 -0.6 1,105,291 + SGL_RS07045 0.51 -0.5 1,105,291 + SGL_RS07045 0.51 -0.5 1,105,454 - SGL_RS07045 0.82 -0.0 1,105,462 + SGL_RS07045 0.84 -0.1 1,105,633 - -0.5 1,105,661 + -0.4 1,105,674 - -0.5 1,105,684 - -0.8 1,105,751 + -0.2 1,105,751 + -0.6 1,105,751 + +1.1 1,105,751 + -0.9 1,105,759 - -1.4 1,105,764 - -1.2 1,106,091 - SGL_RS07050 0.28 -0.7 1,106,292 + SGL_RS07050 0.42 -0.7 1,106,319 - SGL_RS07050 0.43 -0.9 1,106,329 + SGL_RS07050 0.44 -0.6 1,106,362 + SGL_RS07050 0.46 -1.9 1,106,633 + SGL_RS07050 0.64 -0.4 1,106,664 - SGL_RS07050 0.66 +1.5 1,106,689 + SGL_RS07050 0.68 -0.1 1,106,828 - SGL_RS07050 0.77 -2.1 1,106,873 + SGL_RS07050 0.80 +0.5 1,106,914 - SGL_RS07050 0.83 -1.6 1,106,935 - SGL_RS07050 0.84 -0.7 1,106,936 - SGL_RS07050 0.84 -0.8 1,106,997 + SGL_RS07050 0.89 -0.5 1,107,007 + SGL_RS07050 0.89 -1.0 1,107,016 - SGL_RS07050 0.90 -0.8 1,107,065 + +0.4 1,107,163 + -0.2 1,107,171 - -0.5 1,107,181 + +0.4 1,107,181 + -0.2
Or see this region's nucleotide sequence