Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04430 and SGL_RS04435 are separated by 49 nucleotides SGL_RS04435 and SGL_RS04440 are separated by 197 nucleotides SGL_RS04440 and SGL_RS04445 are separated by 94 nucleotides
SGL_RS04430: SGL_RS04430 - CocE/NonD family hydrolase, at 550,533 to 552,170
_RS04430
SGL_RS04435: SGL_RS04435 - FAD-dependent thymidylate synthase, at 552,220 to 552,867
_RS04435
SGL_RS04440: SGL_RS04440 - photosystem II reaction center protein K, at 553,065 to 553,202
_RS04440
SGL_RS04445: SGL_RS04445 - 1-acyl-sn-glycerol-3-phosphate acyltransferase, at 553,297 to 554,019
_RS04445
Position (kb) 552
553 Strain fitness (log2 ratio) -2
-1
0
1
2 at 551.399 kb on + strand, within SGL_RS04430 at 551.439 kb on + strand, within SGL_RS04430 at 551.586 kb on + strand, within SGL_RS04430 at 551.740 kb on + strand, within SGL_RS04430 at 551.763 kb on - strand, within SGL_RS04430 at 551.765 kb on + strand, within SGL_RS04430 at 551.803 kb on + strand, within SGL_RS04430 at 551.803 kb on + strand, within SGL_RS04430 at 551.898 kb on - strand, within SGL_RS04430 at 552.005 kb on + strand, within SGL_RS04430 at 552.047 kb on + strand at 552.047 kb on + strand at 552.055 kb on - strand at 552.105 kb on - strand at 552.129 kb on + strand at 553.340 kb on + strand at 553.348 kb on - strand at 553.370 kb on - strand, within SGL_RS04445 at 553.484 kb on + strand, within SGL_RS04445 at 553.492 kb on - strand, within SGL_RS04445 at 553.508 kb on + strand, within SGL_RS04445 at 553.516 kb on - strand, within SGL_RS04445 at 553.520 kb on + strand, within SGL_RS04445 at 553.576 kb on + strand, within SGL_RS04445 at 553.732 kb on + strand, within SGL_RS04445 at 553.732 kb on + strand, within SGL_RS04445 at 553.751 kb on - strand, within SGL_RS04445 at 553.810 kb on - strand, within SGL_RS04445 at 553.846 kb on + strand, within SGL_RS04445 at 553.849 kb on - strand, within SGL_RS04445
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 551,399 + SGL_RS04430 0.53 -0.6 551,439 + SGL_RS04430 0.55 +0.0 551,586 + SGL_RS04430 0.64 -2.3 551,740 + SGL_RS04430 0.74 -2.7 551,763 - SGL_RS04430 0.75 -1.1 551,765 + SGL_RS04430 0.75 -1.8 551,803 + SGL_RS04430 0.78 -1.9 551,803 + SGL_RS04430 0.78 -1.2 551,898 - SGL_RS04430 0.83 -1.4 552,005 + SGL_RS04430 0.90 -1.5 552,047 + -2.1 552,047 + -0.1 552,055 - -1.6 552,105 - -2.0 552,129 + -2.0 553,340 + +0.8 553,348 - +0.6 553,370 - SGL_RS04445 0.10 +1.5 553,484 + SGL_RS04445 0.26 +0.9 553,492 - SGL_RS04445 0.27 +1.7 553,508 + SGL_RS04445 0.29 +1.1 553,516 - SGL_RS04445 0.30 +0.5 553,520 + SGL_RS04445 0.31 +0.9 553,576 + SGL_RS04445 0.39 +1.1 553,732 + SGL_RS04445 0.60 +0.7 553,732 + SGL_RS04445 0.60 +1.5 553,751 - SGL_RS04445 0.63 +0.4 553,810 - SGL_RS04445 0.71 +2.1 553,846 + SGL_RS04445 0.76 +0.9 553,849 - SGL_RS04445 0.76 +1.4
Or see this region's nucleotide sequence