Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04310 and SGL_RS04315 are separated by 162 nucleotides SGL_RS04315 and SGL_RS04320 are separated by 22 nucleotides SGL_RS04320 and SGL_RS04325 are separated by 288 nucleotides
SGL_RS04310: SGL_RS04310 - MlaD family protein, at 521,483 to 522,853
_RS04310
SGL_RS04315: SGL_RS04315 - ABC transporter ATP-binding protein, at 523,016 to 523,798
_RS04315
SGL_RS04320: SGL_RS04320 - hypothetical protein, at 523,821 to 524,093
_RS04320
SGL_RS04325: SGL_RS04325 - CHAT domain-containing protein, at 524,382 to 529,595
_RS04325
Position (kb) 523
524
525 Strain fitness (log2 ratio) -1
0
1 at 522.846 kb on - strand at 522.852 kb on - strand at 522.873 kb on - strand at 522.970 kb on - strand at 523.194 kb on - strand, within SGL_RS04315 at 523.267 kb on - strand, within SGL_RS04315 at 523.350 kb on + strand, within SGL_RS04315 at 523.397 kb on - strand, within SGL_RS04315 at 523.579 kb on + strand, within SGL_RS04315 at 523.615 kb on + strand, within SGL_RS04315 at 523.723 kb on + strand at 523.723 kb on + strand at 523.731 kb on - strand at 523.757 kb on + strand at 523.762 kb on + strand at 523.783 kb on - strand at 523.918 kb on - strand, within SGL_RS04320 at 523.941 kb on + strand, within SGL_RS04320 at 524.022 kb on + strand, within SGL_RS04320 at 524.317 kb on - strand at 524.459 kb on - strand at 524.480 kb on + strand at 524.599 kb on - strand at 524.715 kb on - strand at 524.850 kb on + strand at 524.937 kb on + strand, within SGL_RS04325
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 522,846 - +0.5 522,852 - -0.8 522,873 - -0.0 522,970 - -0.1 523,194 - SGL_RS04315 0.23 +1.0 523,267 - SGL_RS04315 0.32 +0.3 523,350 + SGL_RS04315 0.43 -0.2 523,397 - SGL_RS04315 0.49 -0.7 523,579 + SGL_RS04315 0.72 +0.7 523,615 + SGL_RS04315 0.77 -0.7 523,723 + -0.3 523,723 + +0.3 523,731 - -0.7 523,757 + -0.7 523,762 + -0.3 523,783 - -0.3 523,918 - SGL_RS04320 0.36 -0.9 523,941 + SGL_RS04320 0.44 -1.0 524,022 + SGL_RS04320 0.74 -0.9 524,317 - +0.3 524,459 - -0.1 524,480 + -0.0 524,599 - +0.1 524,715 - -0.3 524,850 + -0.6 524,937 + SGL_RS04325 0.11 +0.0
Or see this region's nucleotide sequence