Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02955 and SGL_RS02960 overlap by 1 nucleotides SGL_RS02960 and SGL_RS02965 are separated by 161 nucleotides SGL_RS02965 and SGL_RS02970 are separated by 18 nucleotides
SGL_RS02955: SGL_RS02955 - aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme, at 234,905 to 236,356
_RS02955
SGL_RS02960: SGL_RS02960 - alanine dehydrogenase, at 236,356 to 237,438
_RS02960
SGL_RS02965: SGL_RS02965 - pyridoxine 5'-phosphate synthase, at 237,600 to 238,325
_RS02965
SGL_RS02970: SGL_RS02970 - DUF2488 family protein, at 238,344 to 238,745
_RS02970
Position (kb) 236
237
238 Strain fitness (log2 ratio) -2
-1
0
1
2 at 236.104 kb on + strand, within SGL_RS02955 at 236.238 kb on - strand at 236.378 kb on - strand at 236.473 kb on + strand, within SGL_RS02960 at 236.568 kb on - strand, within SGL_RS02960 at 236.692 kb on + strand, within SGL_RS02960 at 236.727 kb on - strand, within SGL_RS02960 at 236.776 kb on + strand, within SGL_RS02960 at 236.799 kb on + strand, within SGL_RS02960 at 236.836 kb on + strand, within SGL_RS02960 at 236.862 kb on + strand, within SGL_RS02960 at 236.862 kb on + strand, within SGL_RS02960 at 236.895 kb on + strand, within SGL_RS02960 at 236.925 kb on - strand, within SGL_RS02960 at 237.008 kb on + strand, within SGL_RS02960 at 237.118 kb on + strand, within SGL_RS02960 at 237.199 kb on + strand, within SGL_RS02960 at 237.236 kb on + strand, within SGL_RS02960 at 237.306 kb on + strand, within SGL_RS02960 at 237.326 kb on - strand, within SGL_RS02960 at 237.374 kb on + strand at 237.672 kb on - strand at 237.682 kb on + strand, within SGL_RS02965 at 237.946 kb on + strand, within SGL_RS02965 at 238.162 kb on + strand, within SGL_RS02965
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 236,104 + SGL_RS02955 0.83 +1.9 236,238 - +2.2 236,378 - -0.6 236,473 + SGL_RS02960 0.11 -0.2 236,568 - SGL_RS02960 0.20 -0.7 236,692 + SGL_RS02960 0.31 -0.4 236,727 - SGL_RS02960 0.34 -0.7 236,776 + SGL_RS02960 0.39 +1.3 236,799 + SGL_RS02960 0.41 -1.2 236,836 + SGL_RS02960 0.44 +0.4 236,862 + SGL_RS02960 0.47 -0.2 236,862 + SGL_RS02960 0.47 -1.0 236,895 + SGL_RS02960 0.50 -0.4 236,925 - SGL_RS02960 0.53 -1.1 237,008 + SGL_RS02960 0.60 -1.1 237,118 + SGL_RS02960 0.70 -0.7 237,199 + SGL_RS02960 0.78 -0.3 237,236 + SGL_RS02960 0.81 -1.8 237,306 + SGL_RS02960 0.88 -2.0 237,326 - SGL_RS02960 0.90 +0.1 237,374 + -0.2 237,672 - +1.3 237,682 + SGL_RS02965 0.11 +2.3 237,946 + SGL_RS02965 0.48 +0.3 238,162 + SGL_RS02965 0.77 +0.3
Or see this region's nucleotide sequence