Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS19390 and SGL_RS01070 are separated by 48 nucleotides SGL_RS01070 and SGL_RS01075 are separated by 18 nucleotides SGL_RS01075 and SGL_RS01080 are separated by 57 nucleotides SGL_RS01080 and SGL_RS01085 are separated by 106 nucleotides
SGL_RS19390: SGL_RS19390 - hypothetical protein, at 93,076 to 93,216
_RS19390
SGL_RS01070: SGL_RS01070 - hypothetical protein, at 93,265 to 93,654
_RS01070
SGL_RS01075: SGL_RS01075 - AAA family ATPase, at 93,673 to 94,188
_RS01075
SGL_RS01080: SGL_RS01080 - hypothetical protein, at 94,246 to 94,611
_RS01080
SGL_RS01085: SGL_RS01085 - alpha-ketoglutarate-dependent dioxygenase AlkB, at 94,718 to 95,344
_RS01085
Position (kb) 93
94
95 Strain fitness (log2 ratio) -1
0
1 at 92.828 kb on - strand at 93.304 kb on - strand, within SGL_RS01070 at 93.331 kb on - strand, within SGL_RS01070 at 93.423 kb on - strand, within SGL_RS01070 at 93.520 kb on - strand, within SGL_RS01070 at 93.575 kb on - strand, within SGL_RS01070 at 93.581 kb on - strand, within SGL_RS01070 at 94.061 kb on + strand, within SGL_RS01075 at 94.285 kb on - strand, within SGL_RS01080 at 94.295 kb on - strand, within SGL_RS01080 at 94.519 kb on - strand, within SGL_RS01080 at 94.599 kb on + strand at 94.819 kb on - strand, within SGL_RS01085 at 94.831 kb on - strand, within SGL_RS01085 at 94.915 kb on - strand, within SGL_RS01085 at 94.935 kb on - strand, within SGL_RS01085 at 94.951 kb on - strand, within SGL_RS01085
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 92,828 - +0.3 93,304 - SGL_RS01070 0.10 +0.1 93,331 - SGL_RS01070 0.17 -0.3 93,423 - SGL_RS01070 0.41 -0.7 93,520 - SGL_RS01070 0.65 +0.2 93,575 - SGL_RS01070 0.79 +0.4 93,581 - SGL_RS01070 0.81 -0.2 94,061 + SGL_RS01075 0.75 -0.7 94,285 - SGL_RS01080 0.11 +0.4 94,295 - SGL_RS01080 0.13 -0.2 94,519 - SGL_RS01080 0.75 -0.0 94,599 + -0.4 94,819 - SGL_RS01085 0.16 -0.5 94,831 - SGL_RS01085 0.18 -0.1 94,915 - SGL_RS01085 0.31 -0.2 94,935 - SGL_RS01085 0.35 +0.3 94,951 - SGL_RS01085 0.37 +0.2
Or see this region's nucleotide sequence