Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01030 and SGL_RS01035 are separated by 38 nucleotides SGL_RS01035 and SGL_RS01040 are separated by 91 nucleotides SGL_RS01040 and SGL_RS01045 are separated by 23 nucleotides
SGL_RS01030: SGL_RS01030 - hypothetical protein, at 82,196 to 82,591
_RS01030
SGL_RS01035: SGL_RS01035 - type III-B CRISPR module RAMP protein Cmr4, at 82,630 to 83,409
_RS01035
SGL_RS01040: SGL_RS01040 - peptidase, at 83,501 to 83,881
_RS01040
SGL_RS01045: SGL_RS01045 - type III-B CRISPR module-associated protein Cmr3, at 83,905 to 85,017
_RS01045
Position (kb) 83
84 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 82.511 kb on + strand, within SGL_RS01030 at 82.511 kb on + strand, within SGL_RS01030 at 82.519 kb on - strand, within SGL_RS01030 at 82.519 kb on - strand, within SGL_RS01030 at 82.535 kb on + strand, within SGL_RS01030 at 82.844 kb on + strand, within SGL_RS01035 at 83.156 kb on + strand, within SGL_RS01035 at 83.218 kb on + strand, within SGL_RS01035 at 83.371 kb on + strand at 83.535 kb on + strand at 83.778 kb on + strand, within SGL_RS01040 at 83.858 kb on - strand at 83.858 kb on - strand at 83.858 kb on - strand at 83.858 kb on - strand at 83.919 kb on + strand at 84.010 kb on - strand at 84.078 kb on + strand, within SGL_RS01045 at 84.180 kb on + strand, within SGL_RS01045 at 84.185 kb on + strand, within SGL_RS01045 at 84.239 kb on + strand, within SGL_RS01045 at 84.239 kb on - strand, within SGL_RS01045 at 84.277 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.316 kb on + strand, within SGL_RS01045 at 84.324 kb on - strand, within SGL_RS01045 at 84.324 kb on - strand, within SGL_RS01045 at 84.369 kb on - strand, within SGL_RS01045 at 84.400 kb on - strand, within SGL_RS01045 at 84.542 kb on + strand, within SGL_RS01045 at 84.542 kb on + strand, within SGL_RS01045 at 84.542 kb on + strand, within SGL_RS01045 at 84.542 kb on + strand, within SGL_RS01045 at 84.550 kb on - strand, within SGL_RS01045 at 84.550 kb on - strand, within SGL_RS01045 at 84.550 kb on - strand, within SGL_RS01045 at 84.550 kb on - strand, within SGL_RS01045 at 84.555 kb on - strand, within SGL_RS01045 at 84.574 kb on + strand, within SGL_RS01045 at 84.641 kb on + strand, within SGL_RS01045 at 84.766 kb on + strand, within SGL_RS01045 at 84.766 kb on + strand, within SGL_RS01045 at 84.857 kb on - strand, within SGL_RS01045
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8 remove 82,511 + SGL_RS01030 0.80 +0.3 82,511 + SGL_RS01030 0.80 -0.5 82,519 - SGL_RS01030 0.82 -0.0 82,519 - SGL_RS01030 0.82 +0.7 82,535 + SGL_RS01030 0.86 +0.2 82,844 + SGL_RS01035 0.27 -0.3 83,156 + SGL_RS01035 0.67 -0.6 83,218 + SGL_RS01035 0.75 -0.1 83,371 + +1.3 83,535 + +0.1 83,778 + SGL_RS01040 0.73 -0.7 83,858 - -0.3 83,858 - -0.4 83,858 - -0.5 83,858 - -0.3 83,919 + +0.2 84,010 - -0.6 84,078 + SGL_RS01045 0.16 +0.6 84,180 + SGL_RS01045 0.25 -0.5 84,185 + SGL_RS01045 0.25 +0.1 84,239 + SGL_RS01045 0.30 +0.1 84,239 - SGL_RS01045 0.30 -0.6 84,277 + SGL_RS01045 0.33 -0.4 84,316 + SGL_RS01045 0.37 +0.4 84,316 + SGL_RS01045 0.37 +0.3 84,316 + SGL_RS01045 0.37 -0.4 84,316 + SGL_RS01045 0.37 -1.5 84,324 - SGL_RS01045 0.38 +0.3 84,324 - SGL_RS01045 0.38 +5.7 84,369 - SGL_RS01045 0.42 -0.4 84,400 - SGL_RS01045 0.44 +0.4 84,542 + SGL_RS01045 0.57 -0.6 84,542 + SGL_RS01045 0.57 -0.7 84,542 + SGL_RS01045 0.57 +0.8 84,542 + SGL_RS01045 0.57 -0.3 84,550 - SGL_RS01045 0.58 +0.4 84,550 - SGL_RS01045 0.58 -0.2 84,550 - SGL_RS01045 0.58 -0.3 84,550 - SGL_RS01045 0.58 -0.3 84,555 - SGL_RS01045 0.58 +1.0 84,574 + SGL_RS01045 0.60 -0.6 84,641 + SGL_RS01045 0.66 +0.4 84,766 + SGL_RS01045 0.77 -0.6 84,766 + SGL_RS01045 0.77 +0.5 84,857 - SGL_RS01045 0.86 -0.6
Or see this region's nucleotide sequence