Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS00120

Experiment: NaNO2 10 mM as N source, buffered with 20 mM TES pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS00110 and SGL_RS00115 overlap by 1 nucleotidesSGL_RS00115 and SGL_RS00120 are separated by 123 nucleotidesSGL_RS00120 and SGL_RS00125 are separated by 42 nucleotides SGL_RS00110: SGL_RS00110 - AAA family ATPase, at 25,994 to 27,175 _RS00110 SGL_RS00115: SGL_RS00115 - DUF4276 family protein, at 27,175 to 27,735 _RS00115 SGL_RS00120: SGL_RS00120 - DUF433 domain-containing protein, at 27,859 to 28,053 _RS00120 SGL_RS00125: SGL_RS00125 - DNA phosphorothioation-associated putative methyltransferase, at 28,096 to 30,168 _RS00125 Position (kb) 27 28 29Strain fitness (log2 ratio) -1 0 1at 26.888 kb on - strand, within SGL_RS00110at 26.965 kb on + strand, within SGL_RS00110at 27.023 kb on + strand, within SGL_RS00110at 27.137 kb on + strandat 27.738 kb on + strandat 28.000 kb on - strand, within SGL_RS00120at 28.000 kb on - strand, within SGL_RS00120at 28.035 kb on + strandat 28.043 kb on - strandat 28.182 kb on + strandat 28.277 kb on + strandat 28.285 kb on - strandat 28.333 kb on - strand, within SGL_RS00125at 28.334 kb on + strand, within SGL_RS00125at 28.358 kb on - strand, within SGL_RS00125at 29.026 kb on + strand, within SGL_RS00125at 29.050 kb on + strand, within SGL_RS00125

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Per-strain Table

Position Strand Gene LocusTag Fraction NaNO2 10 mM as N source, buffered with 20 mM TES pH8
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26,888 - SGL_RS00110 0.76 -0.1
26,965 + SGL_RS00110 0.82 +0.6
27,023 + SGL_RS00110 0.87 -0.4
27,137 + +0.1
27,738 + -1.4
28,000 - SGL_RS00120 0.72 -0.1
28,000 - SGL_RS00120 0.72 -1.7
28,035 + +0.2
28,043 - -0.3
28,182 + -0.0
28,277 + -0.6
28,285 - +0.4
28,333 - SGL_RS00125 0.11 -0.4
28,334 + SGL_RS00125 0.11 -1.0
28,358 - SGL_RS00125 0.13 -0.8
29,026 + SGL_RS00125 0.45 -0.2
29,050 + SGL_RS00125 0.46 +0.0

Or see this region's nucleotide sequence