Experiment: NaNO3 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05435 and SGL_RS05440 are separated by 188 nucleotides SGL_RS05440 and SGL_RS05445 are separated by 281 nucleotides SGL_RS05445 and SGL_RS05450 are separated by 66 nucleotides
SGL_RS05435: SGL_RS05435 - heavy metal translocating P-type ATPase, at 764,301 to 766,538
_RS05435
SGL_RS05440: SGL_RS05440 - ACP S-malonyltransferase, at 766,727 to 767,608
_RS05440
SGL_RS05445: SGL_RS05445 - response regulator, at 767,890 to 768,432
_RS05445
SGL_RS05450: SGL_RS05450 - YbjN domain-containing protein, at 768,499 to 768,960
_RS05450
Position (kb) 766
767
768 Strain fitness (log2 ratio) -2
-1
0
1 at 766.018 kb on - strand, within SGL_RS05435 at 766.364 kb on - strand at 766.490 kb on + strand at 766.538 kb on + strand at 766.558 kb on + strand at 766.889 kb on - strand, within SGL_RS05440 at 767.218 kb on - strand, within SGL_RS05440 at 767.220 kb on - strand, within SGL_RS05440 at 767.223 kb on - strand, within SGL_RS05440 at 767.242 kb on + strand, within SGL_RS05440 at 767.320 kb on - strand, within SGL_RS05440 at 767.708 kb on + strand at 767.743 kb on - strand at 767.792 kb on + strand at 767.831 kb on + strand at 767.831 kb on - strand at 767.850 kb on - strand at 767.979 kb on + strand, within SGL_RS05445 at 768.005 kb on + strand, within SGL_RS05445 at 768.255 kb on - strand, within SGL_RS05445 at 768.525 kb on - strand at 768.565 kb on - strand, within SGL_RS05450
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with 20 mM TES pH8 remove 766,018 - SGL_RS05435 0.77 -2.2 766,364 - -1.6 766,490 + -1.8 766,538 + -0.6 766,558 + -1.2 766,889 - SGL_RS05440 0.18 +1.2 767,218 - SGL_RS05440 0.56 -1.4 767,220 - SGL_RS05440 0.56 -1.2 767,223 - SGL_RS05440 0.56 +0.2 767,242 + SGL_RS05440 0.58 -0.8 767,320 - SGL_RS05440 0.67 -0.8 767,708 + +0.0 767,743 - +0.3 767,792 + +0.8 767,831 + +0.1 767,831 - +0.1 767,850 - -0.6 767,979 + SGL_RS05445 0.16 -0.7 768,005 + SGL_RS05445 0.21 +0.2 768,255 - SGL_RS05445 0.67 -0.5 768,525 - +0.2 768,565 - SGL_RS05450 0.14 -0.0
Or see this region's nucleotide sequence