Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS05005

Experiment: NaNO3 10 mM as N source, buffered with 20 mM TES pH8

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS04995 and SGL_RS05000 are separated by 46 nucleotidesSGL_RS05000 and SGL_RS05005 are separated by 41 nucleotidesSGL_RS05005 and SGL_RS05010 are separated by 276 nucleotides SGL_RS04995: SGL_RS04995 - hypothetical protein, at 671,912 to 672,244 _RS04995 SGL_RS05000: SGL_RS05000 - signal peptidase I, at 672,291 to 672,947 _RS05000 SGL_RS05005: SGL_RS05005 - Uma2 family endonuclease, at 672,989 to 673,612 _RS05005 SGL_RS05010: SGL_RS05010 - cytochrome ubiquinol oxidase subunit I, at 673,889 to 675,340 _RS05010 Position (kb) 672 673 674Strain fitness (log2 ratio) -1 0 1at 672.195 kb on - strand, within SGL_RS04995at 672.848 kb on + strand, within SGL_RS05000at 673.315 kb on + strand, within SGL_RS05005at 673.469 kb on - strand, within SGL_RS05005at 673.509 kb on - strand, within SGL_RS05005at 673.620 kb on - strandat 673.889 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with 20 mM TES pH8
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672,195 - SGL_RS04995 0.85 -0.3
672,848 + SGL_RS05000 0.85 +0.5
673,315 + SGL_RS05005 0.52 -1.1
673,469 - SGL_RS05005 0.77 -0.8
673,509 - SGL_RS05005 0.83 -0.7
673,620 - +0.3
673,889 - +0.2

Or see this region's nucleotide sequence