Experiment: NaNO3 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS04955 and SGL_RS04960 overlap by 17 nucleotides SGL_RS04960 and SGL_RS04965 are separated by 71 nucleotides SGL_RS04965 and SGL_RS04970 are separated by 9 nucleotides
SGL_RS04955: SGL_RS04955 - mechanosensitive ion channel family protein, at 664,903 to 666,945
_RS04955
SGL_RS04960: SGL_RS04960 - VOC family protein, at 666,929 to 667,375
_RS04960
SGL_RS04965: SGL_RS04965 - response regulator, at 667,447 to 667,842
_RS04965
SGL_RS04970: SGL_RS04970 - tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD, at 667,852 to 668,772
_RS04970
Position (kb) 666
667
668 Strain fitness (log2 ratio) -1
0
1 at 666.402 kb on + strand, within SGL_RS04955 at 667.079 kb on - strand, within SGL_RS04960 at 667.131 kb on + strand, within SGL_RS04960 at 667.165 kb on - strand, within SGL_RS04960 at 667.635 kb on + strand, within SGL_RS04965 at 667.880 kb on - strand at 667.960 kb on + strand, within SGL_RS04970 at 667.960 kb on + strand, within SGL_RS04970 at 667.965 kb on + strand, within SGL_RS04970 at 668.000 kb on + strand, within SGL_RS04970 at 668.008 kb on - strand, within SGL_RS04970 at 668.134 kb on - strand, within SGL_RS04970 at 668.195 kb on - strand, within SGL_RS04970 at 668.196 kb on - strand, within SGL_RS04970 at 668.250 kb on - strand, within SGL_RS04970 at 668.260 kb on - strand, within SGL_RS04970 at 668.337 kb on - strand, within SGL_RS04970
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with 20 mM TES pH8 remove 666,402 + SGL_RS04955 0.73 -0.2 667,079 - SGL_RS04960 0.34 -0.2 667,131 + SGL_RS04960 0.45 -0.9 667,165 - SGL_RS04960 0.53 -0.2 667,635 + SGL_RS04965 0.47 +0.3 667,880 - -0.1 667,960 + SGL_RS04970 0.12 +0.9 667,960 + SGL_RS04970 0.12 -0.2 667,965 + SGL_RS04970 0.12 -0.5 668,000 + SGL_RS04970 0.16 -0.1 668,008 - SGL_RS04970 0.17 -0.1 668,134 - SGL_RS04970 0.31 -0.1 668,195 - SGL_RS04970 0.37 -0.5 668,196 - SGL_RS04970 0.37 -0.0 668,250 - SGL_RS04970 0.43 +0.1 668,260 - SGL_RS04970 0.44 -0.2 668,337 - SGL_RS04970 0.53 +0.2
Or see this region's nucleotide sequence