Experiment: NaNO3 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS02505 and SGL_RS02510 are separated by 116 nucleotides SGL_RS02510 and SGL_RS02515 overlap by 1 nucleotides SGL_RS02515 and SGL_RS02520 are separated by 234 nucleotides SGL_RS02520 and SGL_RS02525 are separated by 10 nucleotides
SGL_RS02505: SGL_RS02505 - precorrin-4 C(11)-methyltransferase, at 141,648 to 142,442
_RS02505
SGL_RS02510: SGL_RS02510 - BlaI/MecI/CopY family transcriptional regulator, at 142,559 to 142,981
_RS02510
SGL_RS02515: SGL_RS02515 - M56 family metallopeptidase, at 142,981 to 143,826
_RS02515
SGL_RS02520: SGL_RS02520 - thioredoxin-dependent thiol peroxidase, at 144,061 to 144,543
_RS02520
SGL_RS02525: SGL_RS02525 - hypothetical protein, at 144,554 to 145,081
_RS02525
Position (kb) 142
143
144 Strain fitness (log2 ratio) -2
-1
0
1
2 at 142.158 kb on + strand, within SGL_RS02505 at 142.166 kb on - strand, within SGL_RS02505 at 142.251 kb on - strand, within SGL_RS02505 at 142.336 kb on + strand, within SGL_RS02505 at 142.420 kb on + strand at 142.455 kb on - strand at 142.526 kb on - strand at 142.526 kb on - strand at 142.545 kb on - strand at 142.602 kb on + strand, within SGL_RS02510 at 142.813 kb on + strand, within SGL_RS02510 at 142.894 kb on + strand, within SGL_RS02510 at 142.950 kb on + strand at 143.018 kb on + strand at 143.023 kb on + strand at 143.210 kb on + strand, within SGL_RS02515 at 143.343 kb on + strand, within SGL_RS02515 at 143.357 kb on + strand, within SGL_RS02515 at 143.419 kb on + strand, within SGL_RS02515 at 143.543 kb on + strand, within SGL_RS02515 at 143.664 kb on + strand, within SGL_RS02515 at 143.927 kb on + strand at 144.473 kb on - strand, within SGL_RS02520 at 144.531 kb on - strand at 144.693 kb on - strand, within SGL_RS02525 at 144.766 kb on + strand, within SGL_RS02525 at 144.821 kb on + strand, within SGL_RS02525
Per-strain Table
Position Strand Gene LocusTag Fraction NaNO3 10 mM as N source, buffered with 20 mM TES pH8 remove 142,158 + SGL_RS02505 0.64 -0.1 142,166 - SGL_RS02505 0.65 +0.2 142,251 - SGL_RS02505 0.76 -0.8 142,336 + SGL_RS02505 0.87 +2.2 142,420 + +0.9 142,455 - +0.0 142,526 - -0.4 142,526 - +1.1 142,545 - -0.5 142,602 + SGL_RS02510 0.10 +0.0 142,813 + SGL_RS02510 0.60 +0.1 142,894 + SGL_RS02510 0.79 -0.6 142,950 + +0.5 143,018 + -1.6 143,023 + -0.8 143,210 + SGL_RS02515 0.27 -0.1 143,343 + SGL_RS02515 0.43 -2.3 143,357 + SGL_RS02515 0.44 -0.9 143,419 + SGL_RS02515 0.52 -1.1 143,543 + SGL_RS02515 0.66 -1.1 143,664 + SGL_RS02515 0.81 -1.1 143,927 + +0.7 144,473 - SGL_RS02520 0.85 -0.5 144,531 - -1.1 144,693 - SGL_RS02525 0.26 -0.3 144,766 + SGL_RS02525 0.40 -0.1 144,821 + SGL_RS02525 0.51 +0.0
Or see this region's nucleotide sequence