Experiment: ClNH4 10 mM as N source, buffered with 20 mM TES pH8
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01610 and SGL_RS01615 overlap by 4 nucleotides SGL_RS01615 and SGL_RS01620 are separated by 3 nucleotides SGL_RS01620 and SGL_RS01625 are separated by 102 nucleotides SGL_RS01625 and SGL_RS01630 are separated by 5 nucleotides
SGL_RS01610: SGL_RS01610 - ThiF family adenylyltransferase, at 51,986 to 53,401
_RS01610
SGL_RS01615: SGL_RS01615 - E2/UBC family protein, at 53,398 to 53,778
_RS01615
SGL_RS01620: SGL_RS01620 - multiubiquitin domain-containing protein, at 53,782 to 54,240
_RS01620
SGL_RS01625: SGL_RS01625 - helix-turn-helix transcriptional regulator, at 54,343 to 54,702
_RS01625
SGL_RS01630: SGL_RS01630 - ImmA/IrrE family metallo-endopeptidase, at 54,708 to 55,295
_RS01630
Position (kb) 53
54 Strain fitness (log2 ratio) -1
0
1 at 52.398 kb on - strand, within SGL_RS01610 at 52.580 kb on - strand, within SGL_RS01610 at 52.626 kb on + strand, within SGL_RS01610 at 52.640 kb on + strand, within SGL_RS01610 at 52.729 kb on + strand, within SGL_RS01610 at 52.737 kb on - strand, within SGL_RS01610 at 52.737 kb on - strand, within SGL_RS01610 at 52.758 kb on + strand, within SGL_RS01610 at 52.892 kb on - strand, within SGL_RS01610 at 52.914 kb on + strand, within SGL_RS01610 at 52.969 kb on + strand, within SGL_RS01610 at 52.990 kb on + strand, within SGL_RS01610 at 52.990 kb on + strand, within SGL_RS01610 at 52.990 kb on + strand, within SGL_RS01610 at 52.998 kb on - strand, within SGL_RS01610 at 52.998 kb on - strand, within SGL_RS01610 at 53.015 kb on - strand, within SGL_RS01610 at 53.020 kb on - strand, within SGL_RS01610 at 53.223 kb on - strand, within SGL_RS01610 at 53.313 kb on + strand at 53.371 kb on + strand at 53.418 kb on - strand at 53.499 kb on - strand, within SGL_RS01615 at 53.733 kb on - strand, within SGL_RS01615 at 53.780 kb on + strand at 53.880 kb on - strand, within SGL_RS01620 at 53.903 kb on + strand, within SGL_RS01620 at 53.903 kb on + strand, within SGL_RS01620 at 53.903 kb on + strand, within SGL_RS01620 at 53.909 kb on - strand, within SGL_RS01620 at 53.915 kb on + strand, within SGL_RS01620 at 53.924 kb on + strand, within SGL_RS01620 at 54.062 kb on - strand, within SGL_RS01620 at 54.088 kb on - strand, within SGL_RS01620 at 54.104 kb on + strand, within SGL_RS01620 at 54.105 kb on - strand, within SGL_RS01620 at 54.187 kb on + strand, within SGL_RS01620 at 54.193 kb on - strand, within SGL_RS01620 at 54.201 kb on - strand at 54.215 kb on + strand at 54.382 kb on + strand, within SGL_RS01625 at 54.382 kb on + strand, within SGL_RS01625 at 54.390 kb on - strand, within SGL_RS01625 at 54.390 kb on - strand, within SGL_RS01625 at 54.390 kb on - strand, within SGL_RS01625 at 54.399 kb on - strand, within SGL_RS01625 at 54.472 kb on + strand, within SGL_RS01625 at 54.472 kb on + strand, within SGL_RS01625 at 54.472 kb on + strand, within SGL_RS01625 at 54.472 kb on + strand, within SGL_RS01625 at 54.472 kb on + strand, within SGL_RS01625 at 54.480 kb on - strand, within SGL_RS01625 at 54.493 kb on + strand, within SGL_RS01625 at 54.511 kb on + strand, within SGL_RS01625 at 54.519 kb on - strand, within SGL_RS01625 at 54.534 kb on - strand, within SGL_RS01625 at 54.583 kb on - strand, within SGL_RS01625 at 54.583 kb on - strand, within SGL_RS01625 at 54.606 kb on + strand, within SGL_RS01625 at 54.668 kb on - strand at 54.678 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction ClNH4 10 mM as N source, buffered with 20 mM TES pH8 remove 52,398 - SGL_RS01610 0.29 -1.0 52,580 - SGL_RS01610 0.42 +0.2 52,626 + SGL_RS01610 0.45 +0.1 52,640 + SGL_RS01610 0.46 +0.8 52,729 + SGL_RS01610 0.52 +0.1 52,737 - SGL_RS01610 0.53 -0.0 52,737 - SGL_RS01610 0.53 +0.1 52,758 + SGL_RS01610 0.55 -0.1 52,892 - SGL_RS01610 0.64 +0.2 52,914 + SGL_RS01610 0.66 -0.2 52,969 + SGL_RS01610 0.69 -0.1 52,990 + SGL_RS01610 0.71 +0.1 52,990 + SGL_RS01610 0.71 -0.1 52,990 + SGL_RS01610 0.71 +0.4 52,998 - SGL_RS01610 0.71 -0.0 52,998 - SGL_RS01610 0.71 -0.3 53,015 - SGL_RS01610 0.73 +0.3 53,020 - SGL_RS01610 0.73 -0.1 53,223 - SGL_RS01610 0.87 -0.7 53,313 + +0.2 53,371 + -1.2 53,418 - -0.2 53,499 - SGL_RS01615 0.27 -0.2 53,733 - SGL_RS01615 0.88 -0.4 53,780 + -0.0 53,880 - SGL_RS01620 0.21 +0.8 53,903 + SGL_RS01620 0.26 -0.2 53,903 + SGL_RS01620 0.26 +0.1 53,903 + SGL_RS01620 0.26 -0.0 53,909 - SGL_RS01620 0.28 +0.3 53,915 + SGL_RS01620 0.29 +0.1 53,924 + SGL_RS01620 0.31 -0.1 54,062 - SGL_RS01620 0.61 +0.2 54,088 - SGL_RS01620 0.67 -0.1 54,104 + SGL_RS01620 0.70 +0.4 54,105 - SGL_RS01620 0.70 +0.4 54,187 + SGL_RS01620 0.88 +0.2 54,193 - SGL_RS01620 0.90 +0.2 54,201 - +0.2 54,215 + +0.2 54,382 + SGL_RS01625 0.11 -0.5 54,382 + SGL_RS01625 0.11 -0.3 54,390 - SGL_RS01625 0.13 -0.1 54,390 - SGL_RS01625 0.13 -0.2 54,390 - SGL_RS01625 0.13 +0.0 54,399 - SGL_RS01625 0.16 -0.1 54,472 + SGL_RS01625 0.36 +1.5 54,472 + SGL_RS01625 0.36 -0.2 54,472 + SGL_RS01625 0.36 +0.1 54,472 + SGL_RS01625 0.36 -0.3 54,472 + SGL_RS01625 0.36 -0.2 54,480 - SGL_RS01625 0.38 +0.2 54,493 + SGL_RS01625 0.42 -0.7 54,511 + SGL_RS01625 0.47 -0.1 54,519 - SGL_RS01625 0.49 -0.3 54,534 - SGL_RS01625 0.53 -0.1 54,583 - SGL_RS01625 0.67 -0.1 54,583 - SGL_RS01625 0.67 +0.1 54,606 + SGL_RS01625 0.73 +0.2 54,668 - -0.1 54,678 - +0.7
Or see this region's nucleotide sequence