Experiment: growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05480 and SGL_RS05485 are separated by 60 nucleotides SGL_RS05485 and SGL_RS05490 overlap by 4 nucleotides SGL_RS05490 and SGL_RS05495 are separated by 208 nucleotides SGL_RS05495 and SGL_RS05500 are separated by 128 nucleotides
SGL_RS05480: SGL_RS05480 - FAD-dependent oxidoreductase, at 773,827 to 775,626
_RS05480
SGL_RS05485: SGL_RS05485 - putative toxin-antitoxin system toxin component, PIN family, at 775,687 to 776,061
_RS05485
SGL_RS05490: SGL_RS05490 - hypothetical protein, at 776,058 to 776,342
_RS05490
SGL_RS05495: SGL_RS05495 - hypothetical protein, at 776,551 to 776,937
_RS05495
SGL_RS05500: SGL_RS05500 - ribonuclease R, at 777,066 to 779,411
_RS05500
Position (kb) 776
777 Strain fitness (log2 ratio) -1
0
1 at 775.476 kb on + strand at 775.489 kb on + strand at 775.530 kb on + strand at 775.882 kb on - strand, within SGL_RS05485 at 776.016 kb on - strand, within SGL_RS05485 at 776.152 kb on + strand, within SGL_RS05490 at 776.285 kb on + strand, within SGL_RS05490 at 776.628 kb on + strand, within SGL_RS05495 at 776.628 kb on + strand, within SGL_RS05495 at 776.628 kb on + strand, within SGL_RS05495 at 776.636 kb on - strand, within SGL_RS05495 at 776.641 kb on - strand, within SGL_RS05495 at 776.736 kb on - strand, within SGL_RS05495 at 776.797 kb on + strand, within SGL_RS05495 at 777.055 kb on + strand at 777.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation remove 775,476 + +0.2 775,489 + -0.2 775,530 + +0.1 775,882 - SGL_RS05485 0.52 +0.2 776,016 - SGL_RS05485 0.88 +0.4 776,152 + SGL_RS05490 0.33 +0.9 776,285 + SGL_RS05490 0.80 +0.3 776,628 + SGL_RS05495 0.20 +0.1 776,628 + SGL_RS05495 0.20 -0.2 776,628 + SGL_RS05495 0.20 -0.2 776,636 - SGL_RS05495 0.22 +0.0 776,641 - SGL_RS05495 0.23 +0.2 776,736 - SGL_RS05495 0.48 +0.1 776,797 + SGL_RS05495 0.64 +0.0 777,055 + +0.2 777,247 - -1.0
Or see this region's nucleotide sequence