Experiment: growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00425 and SGL_RS00430 are separated by 371 nucleotides SGL_RS00430 and SGL_RS00435 are separated by 136 nucleotides SGL_RS00435 and SGL_RS00440 are separated by 112 nucleotides SGL_RS00440 and SGL_RS00445 overlap by 4 nucleotides SGL_RS00445 and SGL_RS00450 are separated by 135 nucleotides
SGL_RS00425: SGL_RS00425 - hypothetical protein, at 90,353 to 90,601
_RS00425
SGL_RS00430: SGL_RS00430 - hypothetical protein, at 90,973 to 91,218
_RS00430
SGL_RS00435: SGL_RS00435 - NAD(P)/FAD-dependent oxidoreductase, at 91,355 to 92,692
_RS00435
SGL_RS00440: SGL_RS00440 - type II toxin-antitoxin system VapC family toxin, at 92,805 to 93,236
_RS00440
SGL_RS00445: SGL_RS00445 - type II toxin-antitoxin system prevent-host-death family antitoxin, at 93,233 to 93,520
_RS00445
SGL_RS00450: SGL_RS00450 - recombinase family protein, at 93,656 to 93,865
_RS00450
Position (kb) 91
92
93 Strain fitness (log2 ratio) -1
0
1 at 90.897 kb on + strand at 90.936 kb on - strand at 91.199 kb on - strand at 91.250 kb on - strand at 91.308 kb on - strand at 91.489 kb on + strand, within SGL_RS00435 at 91.489 kb on + strand, within SGL_RS00435 at 91.526 kb on - strand, within SGL_RS00435 at 91.604 kb on - strand, within SGL_RS00435 at 91.840 kb on + strand, within SGL_RS00435 at 92.133 kb on - strand, within SGL_RS00435 at 92.284 kb on + strand, within SGL_RS00435 at 92.294 kb on + strand, within SGL_RS00435 at 92.544 kb on - strand, within SGL_RS00435 at 92.605 kb on + strand at 92.866 kb on - strand, within SGL_RS00440 at 92.866 kb on - strand, within SGL_RS00440 at 92.894 kb on - strand, within SGL_RS00440 at 93.051 kb on + strand, within SGL_RS00440 at 93.113 kb on + strand, within SGL_RS00440 at 93.146 kb on - strand, within SGL_RS00440 at 93.170 kb on - strand, within SGL_RS00440 at 93.400 kb on + strand, within SGL_RS00445 at 93.400 kb on + strand, within SGL_RS00445 at 93.402 kb on + strand, within SGL_RS00445 at 93.435 kb on - strand, within SGL_RS00445
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 3 .M Cl2Co but diluted to prevent light attenuation remove 90,897 + +1.1 90,936 - -0.1 91,199 - +0.7 91,250 - -0.2 91,308 - +0.0 91,489 + SGL_RS00435 0.10 +0.0 91,489 + SGL_RS00435 0.10 +0.3 91,526 - SGL_RS00435 0.13 +0.3 91,604 - SGL_RS00435 0.19 +1.2 91,840 + SGL_RS00435 0.36 -0.7 92,133 - SGL_RS00435 0.58 +0.5 92,284 + SGL_RS00435 0.69 +0.5 92,294 + SGL_RS00435 0.70 +0.7 92,544 - SGL_RS00435 0.89 -0.2 92,605 + +0.2 92,866 - SGL_RS00440 0.14 +0.2 92,866 - SGL_RS00440 0.14 -0.5 92,894 - SGL_RS00440 0.21 -0.4 93,051 + SGL_RS00440 0.57 -0.3 93,113 + SGL_RS00440 0.71 +0.4 93,146 - SGL_RS00440 0.79 -0.4 93,170 - SGL_RS00440 0.84 +0.4 93,400 + SGL_RS00445 0.58 +0.2 93,400 + SGL_RS00445 0.58 +0.0 93,402 + SGL_RS00445 0.59 +0.6 93,435 - SGL_RS00445 0.70 -0.1
Or see this region's nucleotide sequence