Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS01295 and SGL_RS01300 are separated by 43 nucleotides SGL_RS01300 and SGL_RS19695 are separated by 366 nucleotides SGL_RS19695 and SGL_RS19700 are separated by 25 nucleotides SGL_RS19700 and SGL_RS19705 are separated by 162 nucleotides
SGL_RS01295: SGL_RS01295 - nitronate monooxygenase, at 29,006 to 30,040
_RS01295
SGL_RS01300: SGL_RS01300 - hypothetical protein, at 30,084 to 30,587
_RS01300
SGL_RS19695: SGL_RS19695 - GNAT family N-acetyltransferase, at 30,954 to 31,376
_RS19695
SGL_RS19700: SGL_RS19700 - GNAT family N-acetyltransferase, at 31,402 to 31,830
_RS19700
SGL_RS19705: SGL_RS19705 - transglutaminase family protein, at 31,993 to 32,598
_RS19705
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1 at 29.983 kb on - strand at 30.063 kb on + strand at 30.080 kb on + strand at 30.088 kb on - strand at 30.090 kb on + strand at 30.090 kb on - strand at 30.136 kb on + strand, within SGL_RS01300 at 30.217 kb on - strand, within SGL_RS01300 at 30.234 kb on + strand, within SGL_RS01300 at 30.265 kb on + strand, within SGL_RS01300 at 30.291 kb on - strand, within SGL_RS01300 at 30.317 kb on + strand, within SGL_RS01300 at 30.346 kb on + strand, within SGL_RS01300 at 30.402 kb on - strand, within SGL_RS01300 at 30.460 kb on - strand, within SGL_RS01300 at 30.460 kb on - strand, within SGL_RS01300 at 30.470 kb on - strand, within SGL_RS01300 at 30.470 kb on - strand, within SGL_RS01300 at 30.480 kb on - strand, within SGL_RS01300 at 30.496 kb on - strand, within SGL_RS01300 at 30.602 kb on + strand at 30.736 kb on - strand at 30.736 kb on - strand at 30.772 kb on - strand at 30.814 kb on + strand at 30.824 kb on + strand at 30.824 kb on + strand at 30.892 kb on + strand at 30.945 kb on - strand at 30.945 kb on - strand at 30.945 kb on - strand at 30.950 kb on - strand at 31.029 kb on + strand, within SGL_RS19695 at 31.080 kb on + strand, within SGL_RS19695 at 31.154 kb on + strand, within SGL_RS19695 at 31.162 kb on + strand, within SGL_RS19695 at 31.164 kb on + strand, within SGL_RS19695 at 31.234 kb on - strand, within SGL_RS19695 at 31.234 kb on - strand, within SGL_RS19695 at 31.250 kb on + strand, within SGL_RS19695 at 31.278 kb on + strand, within SGL_RS19695 at 31.399 kb on + strand at 31.399 kb on + strand at 31.415 kb on - strand at 31.416 kb on - strand at 31.479 kb on + strand, within SGL_RS19700 at 31.484 kb on + strand, within SGL_RS19700 at 31.492 kb on - strand, within SGL_RS19700 at 31.504 kb on + strand, within SGL_RS19700 at 31.629 kb on + strand, within SGL_RS19700 at 31.636 kb on + strand, within SGL_RS19700 at 31.667 kb on - strand, within SGL_RS19700 at 31.793 kb on + strand at 31.861 kb on + strand at 31.863 kb on - strand at 31.895 kb on + strand at 31.914 kb on - strand at 31.997 kb on + strand at 31.997 kb on + strand at 32.058 kb on - strand, within SGL_RS19705 at 32.203 kb on + strand, within SGL_RS19705 at 32.236 kb on + strand, within SGL_RS19705 at 32.288 kb on + strand, within SGL_RS19705 at 32.309 kb on + strand, within SGL_RS19705 at 32.309 kb on - strand, within SGL_RS19705 at 32.317 kb on - strand, within SGL_RS19705 at 32.325 kb on + strand, within SGL_RS19705 at 32.325 kb on + strand, within SGL_RS19705 at 32.346 kb on - strand, within SGL_RS19705
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 29,983 - -1.1 30,063 + -0.1 30,080 + -0.1 30,088 - -0.9 30,090 + +0.3 30,090 - -0.5 30,136 + SGL_RS01300 0.10 -0.3 30,217 - SGL_RS01300 0.26 -0.4 30,234 + SGL_RS01300 0.30 +0.3 30,265 + SGL_RS01300 0.36 -0.0 30,291 - SGL_RS01300 0.41 -0.4 30,317 + SGL_RS01300 0.46 +0.7 30,346 + SGL_RS01300 0.52 +0.4 30,402 - SGL_RS01300 0.63 -0.8 30,460 - SGL_RS01300 0.75 -0.1 30,460 - SGL_RS01300 0.75 -0.4 30,470 - SGL_RS01300 0.77 +0.1 30,470 - SGL_RS01300 0.77 -0.1 30,480 - SGL_RS01300 0.79 +0.6 30,496 - SGL_RS01300 0.82 -0.0 30,602 + -0.1 30,736 - +0.9 30,736 - -0.2 30,772 - +0.2 30,814 + -0.0 30,824 + -2.5 30,824 + -0.4 30,892 + +1.6 30,945 - +0.6 30,945 - -0.6 30,945 - -0.4 30,950 - +0.7 31,029 + SGL_RS19695 0.18 +0.6 31,080 + SGL_RS19695 0.30 +0.1 31,154 + SGL_RS19695 0.47 +0.1 31,162 + SGL_RS19695 0.49 +0.2 31,164 + SGL_RS19695 0.50 +0.6 31,234 - SGL_RS19695 0.66 +1.7 31,234 - SGL_RS19695 0.66 +0.6 31,250 + SGL_RS19695 0.70 +0.3 31,278 + SGL_RS19695 0.77 +0.1 31,399 + +0.1 31,399 + +0.5 31,415 - +0.6 31,416 - +0.7 31,479 + SGL_RS19700 0.18 -0.4 31,484 + SGL_RS19700 0.19 -0.9 31,492 - SGL_RS19700 0.21 +0.2 31,504 + SGL_RS19700 0.24 -0.1 31,629 + SGL_RS19700 0.53 -0.1 31,636 + SGL_RS19700 0.55 +0.7 31,667 - SGL_RS19700 0.62 -0.3 31,793 + +0.0 31,861 + +0.5 31,863 - +0.2 31,895 + +0.4 31,914 - -0.5 31,997 + -0.8 31,997 + -0.6 32,058 - SGL_RS19705 0.11 -0.9 32,203 + SGL_RS19705 0.35 -0.3 32,236 + SGL_RS19705 0.40 +0.5 32,288 + SGL_RS19705 0.49 +0.4 32,309 + SGL_RS19705 0.52 +0.2 32,309 - SGL_RS19705 0.52 +0.5 32,317 - SGL_RS19705 0.53 +0.1 32,325 + SGL_RS19705 0.55 -0.7 32,325 + SGL_RS19705 0.55 -0.3 32,346 - SGL_RS19705 0.58 +0.3
Or see this region's nucleotide sequence