Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS14350 and SGL_RS14355 are separated by 10 nucleotides SGL_RS14355 and SGL_RS14360 are separated by 511 nucleotides SGL_RS14360 and SGL_RS14365 are separated by 384 nucleotides
SGL_RS14350: SGL_RS14350 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase, at 2,674,736 to 2,676,196
_RS14350
SGL_RS14355: SGL_RS14355 - hypothetical protein, at 2,676,207 to 2,676,425
_RS14355
SGL_RS14360: SGL_RS14360 - carbon-nitrogen hydrolase family protein, at 2,676,937 to 2,677,755
_RS14360
SGL_RS14365: SGL_RS14365 - methionine--tRNA ligase, at 2,678,140 to 2,679,738
_RS14365
Position (kb)
2676
2677
2678 Strain fitness (log2 ratio)
-1
0
1 at 2676.009 kb on + strand, within SGL_RS14350 at 2676.134 kb on - strand at 2676.153 kb on - strand at 2676.153 kb on - strand at 2676.213 kb on - strand at 2676.670 kb on - strand at 2676.894 kb on - strand at 2676.900 kb on + strand at 2676.900 kb on + strand at 2676.908 kb on - strand at 2676.910 kb on + strand at 2676.992 kb on - strand at 2677.055 kb on - strand, within SGL_RS14360 at 2677.088 kb on - strand, within SGL_RS14360 at 2677.183 kb on + strand, within SGL_RS14360 at 2677.200 kb on - strand, within SGL_RS14360 at 2677.256 kb on - strand, within SGL_RS14360 at 2677.420 kb on - strand, within SGL_RS14360 at 2677.441 kb on + strand, within SGL_RS14360 at 2677.462 kb on + strand, within SGL_RS14360 at 2677.470 kb on - strand, within SGL_RS14360 at 2677.532 kb on - strand, within SGL_RS14360 at 2677.678 kb on + strand at 2677.707 kb on + strand at 2677.852 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 2,676,009 + SGL_RS14350 0.87 -0.5 2,676,134 - +0.3 2,676,153 - -0.3 2,676,153 - -0.5 2,676,213 - +0.4 2,676,670 - -0.1 2,676,894 - -0.1 2,676,900 + -1.1 2,676,900 + +0.4 2,676,908 - +1.3 2,676,910 + -0.7 2,676,992 - +0.2 2,677,055 - SGL_RS14360 0.14 +0.5 2,677,088 - SGL_RS14360 0.18 +0.2 2,677,183 + SGL_RS14360 0.30 +0.0 2,677,200 - SGL_RS14360 0.32 -1.4 2,677,256 - SGL_RS14360 0.39 -0.8 2,677,420 - SGL_RS14360 0.59 +1.3 2,677,441 + SGL_RS14360 0.62 -1.5 2,677,462 + SGL_RS14360 0.64 +0.3 2,677,470 - SGL_RS14360 0.65 +0.9 2,677,532 - SGL_RS14360 0.73 +0.5 2,677,678 + -0.2 2,677,707 + +0.1 2,677,852 - -0.5
Or see this region's nucleotide sequence