Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12305 and SGL_RS18965 are separated by 21 nucleotides SGL_RS18965 and SGL_RS12310 are separated by 57 nucleotides SGL_RS12310 and SGL_RS12315 are separated by 220 nucleotides SGL_RS12315 and SGL_RS12320 are separated by 198 nucleotides
SGL_RS12305: SGL_RS12305 - type II secretion system protein, at 2,222,316 to 2,222,876
_RS12305
SGL_RS18965: SGL_RS18965 - hypothetical protein, at 2,222,898 to 2,223,815
_RS18965
SGL_RS12310: SGL_RS12310 - alpha/beta hydrolase, at 2,223,873 to 2,224,568
_RS12310
SGL_RS12315: SGL_RS12315 - hypothetical protein, at 2,224,789 to 2,225,079
_RS12315
SGL_RS12320: SGL_RS12320 - hypothetical protein, at 2,225,278 to 2,225,709
_RS12320
Position (kb)
2223
2224
2225 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2223.279 kb on - strand, within SGL_RS18965 at 2223.311 kb on + strand, within SGL_RS18965 at 2223.311 kb on + strand, within SGL_RS18965 at 2223.321 kb on - strand, within SGL_RS18965 at 2223.396 kb on + strand, within SGL_RS18965 at 2223.443 kb on - strand, within SGL_RS18965 at 2223.551 kb on - strand, within SGL_RS18965 at 2223.782 kb on - strand at 2223.925 kb on - strand at 2223.930 kb on - strand at 2224.095 kb on + strand, within SGL_RS12310 at 2224.148 kb on + strand, within SGL_RS12310 at 2224.211 kb on - strand, within SGL_RS12310 at 2224.324 kb on - strand, within SGL_RS12310 at 2224.334 kb on + strand, within SGL_RS12310 at 2224.337 kb on - strand, within SGL_RS12310 at 2224.354 kb on - strand, within SGL_RS12310 at 2224.355 kb on - strand, within SGL_RS12310 at 2224.476 kb on + strand, within SGL_RS12310 at 2224.677 kb on - strand at 2224.785 kb on - strand at 2224.883 kb on - strand, within SGL_RS12315 at 2224.895 kb on - strand, within SGL_RS12315 at 2224.895 kb on - strand, within SGL_RS12315 at 2225.164 kb on - strand at 2225.186 kb on + strand at 2225.349 kb on - strand, within SGL_RS12320 at 2225.420 kb on + strand, within SGL_RS12320 at 2225.428 kb on - strand, within SGL_RS12320 at 2225.514 kb on + strand, within SGL_RS12320
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 2,223,279 - SGL_RS18965 0.42 -0.2 2,223,311 + SGL_RS18965 0.45 +0.1 2,223,311 + SGL_RS18965 0.45 +0.0 2,223,321 - SGL_RS18965 0.46 +0.1 2,223,396 + SGL_RS18965 0.54 +0.3 2,223,443 - SGL_RS18965 0.59 -0.0 2,223,551 - SGL_RS18965 0.71 -0.3 2,223,782 - -0.7 2,223,925 - +0.5 2,223,930 - +0.2 2,224,095 + SGL_RS12310 0.32 +0.5 2,224,148 + SGL_RS12310 0.40 +0.6 2,224,211 - SGL_RS12310 0.49 -0.6 2,224,324 - SGL_RS12310 0.65 -0.1 2,224,334 + SGL_RS12310 0.66 +0.1 2,224,337 - SGL_RS12310 0.67 +1.1 2,224,354 - SGL_RS12310 0.69 +0.2 2,224,355 - SGL_RS12310 0.69 +2.9 2,224,476 + SGL_RS12310 0.87 -0.4 2,224,677 - +0.1 2,224,785 - +0.2 2,224,883 - SGL_RS12315 0.32 -0.5 2,224,895 - SGL_RS12315 0.36 +0.5 2,224,895 - SGL_RS12315 0.36 +0.4 2,225,164 - -0.4 2,225,186 + +0.5 2,225,349 - SGL_RS12320 0.16 +0.1 2,225,420 + SGL_RS12320 0.33 -0.8 2,225,428 - SGL_RS12320 0.35 +0.3 2,225,514 + SGL_RS12320 0.55 -0.2
Or see this region's nucleotide sequence