Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09725 and SGL_RS09730 are separated by 89 nucleotides SGL_RS09730 and SGL_RS09735 are separated by 9 nucleotides SGL_RS09735 and SGL_RS09740 are separated by 21 nucleotides SGL_RS09740 and SGL_RS09745 are separated by 45 nucleotides
SGL_RS09725: SGL_RS09725 - Ni/Fe hydrogenase subunit alpha, at 1,672,071 to 1,673,495
_RS09725
SGL_RS09730: SGL_RS09730 - type II toxin-antitoxin system VapC family toxin, at 1,673,585 to 1,674,034
_RS09730
SGL_RS09735: SGL_RS09735 - hypothetical protein, at 1,674,044 to 1,674,328
_RS09735
SGL_RS09740: SGL_RS09740 - oxidoreductase, at 1,674,350 to 1,674,898
_RS09740
SGL_RS09745: SGL_RS09745 - bidirectional hydrogenase complex protein HoxU, at 1,674,944 to 1,675,660
_RS09745
Position (kb) 1673
1674
1675 Strain fitness (log2 ratio) -1
0
1 at 1672.655 kb on - strand, within SGL_RS09725 at 1672.943 kb on - strand, within SGL_RS09725 at 1672.991 kb on - strand, within SGL_RS09725 at 1672.991 kb on - strand, within SGL_RS09725 at 1673.108 kb on - strand, within SGL_RS09725 at 1673.177 kb on + strand, within SGL_RS09725 at 1673.354 kb on - strand at 1673.423 kb on - strand at 1673.782 kb on + strand, within SGL_RS09730 at 1674.375 kb on - strand at 1674.559 kb on + strand, within SGL_RS09740 at 1675.022 kb on + strand, within SGL_RS09745
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 1,672,655 - SGL_RS09725 0.41 -0.5 1,672,943 - SGL_RS09725 0.61 +0.1 1,672,991 - SGL_RS09725 0.65 +0.2 1,672,991 - SGL_RS09725 0.65 +0.3 1,673,108 - SGL_RS09725 0.73 -0.9 1,673,177 + SGL_RS09725 0.78 +0.3 1,673,354 - -0.3 1,673,423 - +0.5 1,673,782 + SGL_RS09730 0.44 +0.6 1,674,375 - -0.1 1,674,559 + SGL_RS09740 0.38 +0.0 1,675,022 + SGL_RS09745 0.11 +0.1
Or see this region's nucleotide sequence