Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09605 and SGL_RS09610 are separated by 73 nucleotides SGL_RS09610 and SGL_RS09615 are separated by 41 nucleotides SGL_RS09615 and SGL_RS09620 are separated by 17 nucleotides SGL_RS09620 and SGL_RS09625 are separated by 1 nucleotides SGL_RS09625 and SGL_RS09630 overlap by 8 nucleotides
SGL_RS09605: SGL_RS09605 - efflux RND transporter permease subunit, at 1,644,905 to 1,648,090
_RS09605
SGL_RS09610: SGL_RS09610 - hypothetical protein, at 1,648,164 to 1,648,337
_RS09610
SGL_RS09615: SGL_RS09615 - PIN domain-containing protein, at 1,648,379 to 1,648,663
_RS09615
SGL_RS09620: SGL_RS09620 - hypothetical protein, at 1,648,681 to 1,649,439
_RS09620
SGL_RS09625: SGL_RS09625 - hypothetical protein, at 1,649,441 to 1,649,914
_RS09625
SGL_RS09630: SGL_RS09630 - hypothetical protein, at 1,649,907 to 1,650,443
_RS09630
Position (kb)
1648
1649
1650 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1647.754 kb on + strand, within SGL_RS09605 at 1647.968 kb on + strand at 1647.976 kb on - strand at 1648.002 kb on + strand at 1648.027 kb on + strand at 1648.033 kb on + strand at 1648.034 kb on - strand at 1648.081 kb on - strand at 1648.169 kb on + strand at 1648.299 kb on - strand, within SGL_RS09610 at 1648.658 kb on + strand at 1648.666 kb on - strand at 1648.666 kb on - strand at 1648.882 kb on + strand, within SGL_RS09620 at 1650.234 kb on - strand, within SGL_RS09630
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 1,647,754 + SGL_RS09605 0.89 +0.6 1,647,968 + +0.4 1,647,976 - +0.1 1,648,002 + -0.7 1,648,027 + +3.2 1,648,033 + +1.2 1,648,034 - -0.1 1,648,081 - -0.5 1,648,169 + +0.4 1,648,299 - SGL_RS09610 0.78 -0.8 1,648,658 + -0.3 1,648,666 - +0.0 1,648,666 - +0.6 1,648,882 + SGL_RS09620 0.26 +0.2 1,650,234 - SGL_RS09630 0.61 -0.1
Or see this region's nucleotide sequence