Strain Fitness in Synechocystis sp000284455 PCC 6803 around SGL_RS07225

Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntSGL_RS07215 and SGL_RS07225 are separated by 623 nucleotidesSGL_RS07225 and SGL_RS07230 are separated by 173 nucleotidesSGL_RS07230 and SGL_RS07235 are separated by 54 nucleotides SGL_RS07215: SGL_RS07215 - type I glyceraldehyde-3-phosphate dehydrogenase, at 1,147,079 to 1,148,098 _RS07215 SGL_RS07225: SGL_RS07225 - Uma2 family endonuclease, at 1,148,722 to 1,149,297 _RS07225 SGL_RS07230: SGL_RS07230 - leucyl/phenylalanyl-tRNA--protein transferase, at 1,149,471 to 1,150,067 _RS07230 SGL_RS07235: SGL_RS07235 - lipoyl synthase, at 1,150,122 to 1,151,033 _RS07235 Position (kb) 1148 1149 1150Strain fitness (log2 ratio) -1 0 1at 1147.728 kb on - strand, within SGL_RS07215at 1148.283 kb on + strandat 1148.295 kb on + strandat 1148.303 kb on + strandat 1149.176 kb on - strand, within SGL_RS07225at 1149.768 kb on - strand, within SGL_RS07230at 1149.809 kb on + strand, within SGL_RS07230at 1149.965 kb on - strand, within SGL_RS07230

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Per-strain Table

Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
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1,147,728 - SGL_RS07215 0.64 +1.1
1,148,283 + -0.6
1,148,295 + +0.1
1,148,303 + -0.3
1,149,176 - SGL_RS07225 0.79 +0.4
1,149,768 - SGL_RS07230 0.50 +0.3
1,149,809 + SGL_RS07230 0.57 -0.2
1,149,965 - SGL_RS07230 0.83 -0.1

Or see this region's nucleotide sequence