Experiment: growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS03210 and SGL_RS03215 are separated by 20 nucleotides SGL_RS03215 and SGL_RS03220 are separated by 63 nucleotides SGL_RS03220 and SGL_RS03225 are separated by 225 nucleotides
SGL_RS03210: SGL_RS03210 - NADP-dependent isocitrate dehydrogenase, at 283,060 to 284,487
_RS03210
SGL_RS03215: SGL_RS03215 - Uma2 family endonuclease, at 284,508 to 285,080
_RS03215
SGL_RS03220: SGL_RS03220 - Rpn family recombination-promoting nuclease/putative transposase, at 285,144 to 285,938
_RS03220
SGL_RS03225: SGL_RS03225 - NAD(P)H-quinone oxidoreductase subunit 4, at 286,164 to 287,843
_RS03225
Position (kb)
285
286 Strain fitness (log2 ratio)
-1
0
1 at 284.238 kb on + strand, within SGL_RS03210 at 284.296 kb on - strand, within SGL_RS03210 at 284.519 kb on + strand at 284.527 kb on - strand at 284.612 kb on + strand, within SGL_RS03215 at 284.758 kb on - strand, within SGL_RS03215 at 284.761 kb on + strand, within SGL_RS03215 at 284.787 kb on - strand, within SGL_RS03215 at 284.905 kb on - strand, within SGL_RS03215 at 284.918 kb on - strand, within SGL_RS03215 at 285.033 kb on + strand at 285.133 kb on - strand at 285.360 kb on + strand, within SGL_RS03220 at 285.370 kb on + strand, within SGL_RS03220 at 285.380 kb on + strand, within SGL_RS03220 at 285.380 kb on + strand, within SGL_RS03220 at 286.017 kb on - strand at 286.018 kb on - strand at 286.281 kb on - strand at 286.428 kb on + strand, within SGL_RS03225 at 286.428 kb on + strand, within SGL_RS03225 at 286.565 kb on - strand, within SGL_RS03225 at 286.723 kb on - strand, within SGL_RS03225 at 286.732 kb on + strand, within SGL_RS03225 at 286.745 kb on + strand, within SGL_RS03225 at 286.751 kb on - strand, within SGL_RS03225 at 286.763 kb on - strand, within SGL_RS03225 at 286.794 kb on - strand, within SGL_RS03225 at 286.809 kb on + strand, within SGL_RS03225 at 286.886 kb on + strand, within SGL_RS03225
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 2mM sodum metaarsenite but diluted to prevent light attenuation remove 284,238 + SGL_RS03210 0.82 -1.0 284,296 - SGL_RS03210 0.87 -1.0 284,519 + +0.2 284,527 - +0.1 284,612 + SGL_RS03215 0.18 -0.1 284,758 - SGL_RS03215 0.44 -0.2 284,761 + SGL_RS03215 0.44 -0.1 284,787 - SGL_RS03215 0.49 +0.1 284,905 - SGL_RS03215 0.69 +0.4 284,918 - SGL_RS03215 0.72 -0.2 285,033 + -0.5 285,133 - +0.3 285,360 + SGL_RS03220 0.27 +0.2 285,370 + SGL_RS03220 0.28 +0.7 285,380 + SGL_RS03220 0.30 +0.2 285,380 + SGL_RS03220 0.30 +0.4 286,017 - +0.4 286,018 - +0.2 286,281 - +0.6 286,428 + SGL_RS03225 0.16 -0.0 286,428 + SGL_RS03225 0.16 +0.3 286,565 - SGL_RS03225 0.24 -0.1 286,723 - SGL_RS03225 0.33 -0.1 286,732 + SGL_RS03225 0.34 -0.3 286,745 + SGL_RS03225 0.35 +0.3 286,751 - SGL_RS03225 0.35 +0.8 286,763 - SGL_RS03225 0.36 +0.2 286,794 - SGL_RS03225 0.38 +0.5 286,809 + SGL_RS03225 0.38 +0.1 286,886 + SGL_RS03225 0.43 -0.4
Or see this region's nucleotide sequence