Experiment: growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS00695 and SGL_RS00700 overlap by 8 nucleotides SGL_RS00700 and SGL_RS00705 are separated by 9 nucleotides SGL_RS00705 and SGL_RS00710 are separated by 31 nucleotides
SGL_RS00695: SGL_RS00695 - CRISPR-associated endoribonuclease Cas6, at 13,441 to 14,229
_RS00695
SGL_RS00700: SGL_RS00700 - CRISPR-associated protein Cas4, at 14,222 to 14,794
_RS00700
SGL_RS00705: SGL_RS00705 - type I-D CRISPR-associated endonuclease Cas1d, at 14,804 to 15,781
_RS00705
SGL_RS00710: SGL_RS00710 - CRISPR-associated endonuclease Cas2, at 15,813 to 16,097
_RS00710
Position (kb) 14
15
16 Strain fitness (log2 ratio) -1
0
1 at 14.418 kb on - strand, within SGL_RS00700 at 14.650 kb on - strand, within SGL_RS00700 at 14.910 kb on + strand, within SGL_RS00705 at 14.922 kb on + strand, within SGL_RS00705 at 14.969 kb on + strand, within SGL_RS00705 at 14.995 kb on - strand, within SGL_RS00705 at 15.065 kb on + strand, within SGL_RS00705 at 15.096 kb on + strand, within SGL_RS00705 at 15.207 kb on - strand, within SGL_RS00705 at 15.245 kb on - strand, within SGL_RS00705 at 15.255 kb on + strand, within SGL_RS00705 at 15.255 kb on + strand, within SGL_RS00705 at 15.257 kb on + strand, within SGL_RS00705 at 15.263 kb on - strand, within SGL_RS00705 at 15.263 kb on - strand, within SGL_RS00705 at 15.343 kb on + strand, within SGL_RS00705 at 15.351 kb on - strand, within SGL_RS00705 at 15.373 kb on - strand, within SGL_RS00705 at 15.391 kb on + strand, within SGL_RS00705 at 15.399 kb on - strand, within SGL_RS00705 at 15.411 kb on - strand, within SGL_RS00705 at 15.421 kb on - strand, within SGL_RS00705 at 15.426 kb on - strand, within SGL_RS00705 at 15.450 kb on - strand, within SGL_RS00705 at 15.696 kb on + strand at 16.280 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 300 .M BCSA to remove any trace of Cu but diluted to prevent light attenuation remove 14,418 - SGL_RS00700 0.34 -1.1 14,650 - SGL_RS00700 0.75 -0.0 14,910 + SGL_RS00705 0.11 +0.1 14,922 + SGL_RS00705 0.12 +0.6 14,969 + SGL_RS00705 0.17 +0.2 14,995 - SGL_RS00705 0.20 -0.3 15,065 + SGL_RS00705 0.27 +0.3 15,096 + SGL_RS00705 0.30 -0.9 15,207 - SGL_RS00705 0.41 +0.4 15,245 - SGL_RS00705 0.45 -0.7 15,255 + SGL_RS00705 0.46 -0.1 15,255 + SGL_RS00705 0.46 +0.1 15,257 + SGL_RS00705 0.46 +1.2 15,263 - SGL_RS00705 0.47 -0.3 15,263 - SGL_RS00705 0.47 +0.1 15,343 + SGL_RS00705 0.55 +0.0 15,351 - SGL_RS00705 0.56 +0.2 15,373 - SGL_RS00705 0.58 +0.3 15,391 + SGL_RS00705 0.60 +0.0 15,399 - SGL_RS00705 0.61 +0.3 15,411 - SGL_RS00705 0.62 +0.1 15,421 - SGL_RS00705 0.63 +0.2 15,426 - SGL_RS00705 0.64 +0.5 15,450 - SGL_RS00705 0.66 +0.2 15,696 + -0.5 16,280 - -0.2
Or see this region's nucleotide sequence