Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS16050 and SGL_RS16055 overlap by 4 nucleotides SGL_RS16055 and SGL_RS16060 are separated by 121 nucleotides SGL_RS16060 and SGL_RS16065 are separated by 38 nucleotides
SGL_RS16050: SGL_RS16050 - two-component system sensor histidine kinase RppB, at 3,048,902 to 3,050,266
_RS16050
SGL_RS16055: SGL_RS16055 - two-component system response regulator RppA, at 3,050,263 to 3,050,967
_RS16055
SGL_RS16060: SGL_RS16060 - efflux RND transporter periplasmic adaptor subunit, at 3,051,089 to 3,051,733
_RS16060
SGL_RS16065: SGL_RS16065 - CusA/CzcA family heavy metal efflux RND transporter, at 3,051,772 to 3,054,936
_RS16065
Position (kb)
3050
3051 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 3049.273 kb on - strand, within SGL_RS16050 at 3049.444 kb on - strand, within SGL_RS16050 at 3049.470 kb on + strand, within SGL_RS16050 at 3049.478 kb on - strand, within SGL_RS16050 at 3049.480 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.490 kb on - strand, within SGL_RS16050 at 3049.522 kb on - strand, within SGL_RS16050 at 3049.543 kb on - strand, within SGL_RS16050 at 3049.547 kb on + strand, within SGL_RS16050 at 3049.555 kb on - strand, within SGL_RS16050 at 3049.573 kb on - strand, within SGL_RS16050 at 3049.651 kb on - strand, within SGL_RS16050 at 3049.724 kb on + strand, within SGL_RS16050 at 3049.838 kb on - strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.991 kb on + strand, within SGL_RS16050 at 3049.992 kb on + strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3049.995 kb on - strand, within SGL_RS16050 at 3050.000 kb on - strand, within SGL_RS16050 at 3050.067 kb on + strand, within SGL_RS16050 at 3050.067 kb on - strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.083 kb on + strand, within SGL_RS16050 at 3050.137 kb on - strand at 3050.150 kb on - strand at 3050.150 kb on - strand at 3050.178 kb on - strand at 3050.183 kb on - strand at 3050.193 kb on - strand at 3050.268 kb on + strand at 3050.287 kb on + strand at 3050.378 kb on - strand, within SGL_RS16055 at 3050.439 kb on + strand, within SGL_RS16055 at 3050.552 kb on - strand, within SGL_RS16055 at 3050.564 kb on - strand, within SGL_RS16055 at 3050.570 kb on - strand, within SGL_RS16055 at 3050.619 kb on - strand, within SGL_RS16055 at 3050.621 kb on - strand, within SGL_RS16055 at 3050.755 kb on - strand, within SGL_RS16055 at 3050.786 kb on - strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.824 kb on + strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.832 kb on - strand, within SGL_RS16055 at 3050.892 kb on - strand, within SGL_RS16055 at 3051.044 kb on - strand at 3051.044 kb on - strand at 3051.083 kb on + strand at 3051.275 kb on - strand, within SGL_RS16060 at 3051.285 kb on - strand, within SGL_RS16060 at 3051.352 kb on + strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.403 kb on - strand, within SGL_RS16060 at 3051.505 kb on + strand, within SGL_RS16060 at 3051.586 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.651 kb on + strand, within SGL_RS16060 at 3051.659 kb on - strand, within SGL_RS16060 at 3051.828 kb on - strand at 3051.832 kb on - strand at 3051.872 kb on - strand at 3051.887 kb on + strand at 3051.955 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 3,049,273 - SGL_RS16050 0.27 +5.3 3,049,444 - SGL_RS16050 0.40 +5.4 3,049,470 + SGL_RS16050 0.42 +5.1 3,049,478 - SGL_RS16050 0.42 +4.8 3,049,480 - SGL_RS16050 0.42 +5.3 3,049,490 - SGL_RS16050 0.43 +5.3 3,049,490 - SGL_RS16050 0.43 +5.1 3,049,522 - SGL_RS16050 0.45 +4.8 3,049,543 - SGL_RS16050 0.47 +5.2 3,049,547 + SGL_RS16050 0.47 +5.2 3,049,555 - SGL_RS16050 0.48 +5.1 3,049,573 - SGL_RS16050 0.49 +5.1 3,049,651 - SGL_RS16050 0.55 +5.6 3,049,724 + SGL_RS16050 0.60 +5.2 3,049,838 - SGL_RS16050 0.69 +5.2 3,049,991 + SGL_RS16050 0.80 +5.4 3,049,991 + SGL_RS16050 0.80 +5.1 3,049,992 + SGL_RS16050 0.80 +5.2 3,049,995 - SGL_RS16050 0.80 +5.3 3,049,995 - SGL_RS16050 0.80 +5.4 3,050,000 - SGL_RS16050 0.80 +4.9 3,050,067 + SGL_RS16050 0.85 +5.2 3,050,067 - SGL_RS16050 0.85 +4.5 3,050,083 + SGL_RS16050 0.87 +5.5 3,050,083 + SGL_RS16050 0.87 +5.1 3,050,083 + SGL_RS16050 0.87 +5.3 3,050,137 - +5.1 3,050,150 - +5.4 3,050,150 - +5.3 3,050,178 - +6.1 3,050,183 - +5.5 3,050,193 - +5.2 3,050,268 + +3.5 3,050,287 + +5.1 3,050,378 - SGL_RS16055 0.16 +4.5 3,050,439 + SGL_RS16055 0.25 +5.0 3,050,552 - SGL_RS16055 0.41 +5.3 3,050,564 - SGL_RS16055 0.43 +5.4 3,050,570 - SGL_RS16055 0.44 +5.0 3,050,619 - SGL_RS16055 0.50 +5.3 3,050,621 - SGL_RS16055 0.51 +5.2 3,050,755 - SGL_RS16055 0.70 +5.2 3,050,786 - SGL_RS16055 0.74 +4.6 3,050,824 + SGL_RS16055 0.80 +5.1 3,050,824 + SGL_RS16055 0.80 +5.1 3,050,832 - SGL_RS16055 0.81 +5.3 3,050,832 - SGL_RS16055 0.81 +5.0 3,050,892 - SGL_RS16055 0.89 +5.3 3,051,044 - +5.3 3,051,044 - +5.0 3,051,083 + +5.4 3,051,275 - SGL_RS16060 0.29 +5.5 3,051,285 - SGL_RS16060 0.30 +5.4 3,051,352 + SGL_RS16060 0.41 +5.4 3,051,403 - SGL_RS16060 0.49 +5.5 3,051,403 - SGL_RS16060 0.49 +5.3 3,051,505 + SGL_RS16060 0.64 +4.9 3,051,586 + SGL_RS16060 0.77 +5.1 3,051,651 + SGL_RS16060 0.87 +5.5 3,051,651 + SGL_RS16060 0.87 +5.6 3,051,659 - SGL_RS16060 0.88 +5.4 3,051,828 - +1.9 3,051,832 - +1.9 3,051,872 - +1.9 3,051,887 + +2.0 3,051,955 - +1.0
Or see this region's nucleotide sequence