Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS13210 and SGL_RS13215 are separated by 127 nucleotides SGL_RS13215 and SGL_RS19625 are separated by 36 nucleotides SGL_RS19625 and SGL_RS13220 are separated by 6 nucleotides SGL_RS13220 and SGL_RS13225 are separated by 20 nucleotides
SGL_RS13210: SGL_RS13210 - glycosyltransferase, at 2,429,543 to 2,430,964
_RS13210
SGL_RS13215: SGL_RS13215 - SxtJ family membrane protein, at 2,431,092 to 2,431,499
_RS13215
SGL_RS19625: SGL_RS19625 - DUF5989 family protein, at 2,431,536 to 2,431,697
_RS19625
SGL_RS13220: SGL_RS13220 - ribonuclease III, at 2,431,704 to 2,432,438
_RS13220
SGL_RS13225: SGL_RS13225 - carbamoyltransferase HypF, at 2,432,459 to 2,434,762
_RS13225
Position (kb)
2431
2432
2433 Strain fitness (log2 ratio)
-1
0
1
2 at 2431.189 kb on - strand, within SGL_RS13215 at 2431.285 kb on - strand, within SGL_RS13215 at 2431.803 kb on + strand, within SGL_RS13220 at 2431.816 kb on - strand, within SGL_RS13220 at 2431.875 kb on + strand, within SGL_RS13220 at 2431.909 kb on - strand, within SGL_RS13220 at 2431.924 kb on - strand, within SGL_RS13220 at 2431.970 kb on - strand, within SGL_RS13220 at 2432.003 kb on - strand, within SGL_RS13220 at 2432.080 kb on - strand, within SGL_RS13220 at 2432.084 kb on + strand, within SGL_RS13220 at 2432.092 kb on + strand, within SGL_RS13220 at 2432.092 kb on - strand, within SGL_RS13220 at 2432.868 kb on - strand, within SGL_RS13225
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 2,431,189 - SGL_RS13215 0.24 +0.4 2,431,285 - SGL_RS13215 0.47 -0.2 2,431,803 + SGL_RS13220 0.13 +1.1 2,431,816 - SGL_RS13220 0.15 +1.9 2,431,875 + SGL_RS13220 0.23 +0.2 2,431,909 - SGL_RS13220 0.28 +0.3 2,431,924 - SGL_RS13220 0.30 +0.3 2,431,970 - SGL_RS13220 0.36 +0.5 2,432,003 - SGL_RS13220 0.41 +0.7 2,432,080 - SGL_RS13220 0.51 +1.0 2,432,084 + SGL_RS13220 0.52 +1.1 2,432,092 + SGL_RS13220 0.53 +0.7 2,432,092 - SGL_RS13220 0.53 +0.5 2,432,868 - SGL_RS13225 0.18 +0.2
Or see this region's nucleotide sequence