Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS12605 and SGL_RS12610 are separated by 188 nucleotides SGL_RS12610 and SGL_RS12615 are separated by 51 nucleotides SGL_RS12615 and SGL_RS12620 are separated by 266 nucleotides SGL_RS12620 and SGL_RS12625 are separated by 368 nucleotides
SGL_RS12605: SGL_RS12605 - NYN domain-containing protein, at 2,285,410 to 2,285,997
_RS12605
SGL_RS12610: SGL_RS12610 - Uma2 family endonuclease, at 2,286,186 to 2,286,746
_RS12610
SGL_RS12615: SGL_RS12615 - helix-turn-helix transcriptional regulator, at 2,286,798 to 2,287,067
_RS12615
SGL_RS12620: SGL_RS12620 - photosystem I iron-sulfur center protein PsaC, at 2,287,334 to 2,287,579
_RS12620
SGL_RS12625: SGL_RS12625 - NAD(P)H-quinone oxidoreductase subunit 4, at 2,287,948 to 2,289,525
_RS12625
Position (kb)
2286
2287
2288 Strain fitness (log2 ratio)
-1
0
1 at 2285.817 kb on - strand, within SGL_RS12605 at 2285.850 kb on - strand, within SGL_RS12605 at 2285.866 kb on - strand, within SGL_RS12605 at 2285.891 kb on + strand, within SGL_RS12605 at 2285.896 kb on + strand, within SGL_RS12605 at 2285.973 kb on - strand at 2286.105 kb on - strand at 2286.189 kb on - strand at 2286.250 kb on + strand, within SGL_RS12610 at 2286.974 kb on + strand, within SGL_RS12615 at 2286.997 kb on - strand, within SGL_RS12615
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 2,285,817 - SGL_RS12605 0.69 +0.1 2,285,850 - SGL_RS12605 0.75 -0.6 2,285,866 - SGL_RS12605 0.78 +0.1 2,285,891 + SGL_RS12605 0.82 -0.1 2,285,896 + SGL_RS12605 0.83 -0.2 2,285,973 - -0.2 2,286,105 - +0.1 2,286,189 - -0.7 2,286,250 + SGL_RS12610 0.11 +0.1 2,286,974 + SGL_RS12615 0.65 -0.3 2,286,997 - SGL_RS12615 0.74 +1.6
Or see this region's nucleotide sequence