Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09550 and SGL_RS09555 overlap by 4 nucleotides SGL_RS09555 and SGL_RS09560 are separated by 2 nucleotides SGL_RS09560 and SGL_RS09565 are separated by 21 nucleotides SGL_RS09565 and SGL_RS09570 are separated by 119 nucleotides
SGL_RS09550: SGL_RS09550 - acylneuraminate cytidylyltransferase, at 1,635,072 to 1,635,749
_RS09550
SGL_RS09555: SGL_RS09555 - phosphoglycerate dehydrogenase, at 1,635,746 to 1,636,702
_RS09555
SGL_RS09560: SGL_RS09560 - SDR family oxidoreductase, at 1,636,705 to 1,637,454
_RS09560
SGL_RS09565: SGL_RS09565 - DUF115 domain-containing protein, at 1,637,476 to 1,638,270
_RS09565
SGL_RS09570: SGL_RS09570 - glycosyltransferase family 2 protein, at 1,638,390 to 1,639,247
_RS09570
Position (kb) 1636
1637
1638 Strain fitness (log2 ratio) -1
0
1
2 at 1635.782 kb on + strand at 1635.789 kb on + strand at 1635.790 kb on - strand at 1635.797 kb on - strand at 1635.797 kb on - strand at 1635.985 kb on + strand, within SGL_RS09555 at 1636.164 kb on - strand, within SGL_RS09555 at 1636.223 kb on - strand, within SGL_RS09555 at 1636.248 kb on + strand, within SGL_RS09555 at 1636.283 kb on + strand, within SGL_RS09555 at 1636.320 kb on + strand, within SGL_RS09555 at 1636.328 kb on - strand, within SGL_RS09555 at 1636.349 kb on - strand, within SGL_RS09555 at 1636.371 kb on + strand, within SGL_RS09555 at 1636.392 kb on + strand, within SGL_RS09555 at 1636.402 kb on + strand, within SGL_RS09555 at 1636.434 kb on - strand, within SGL_RS09555 at 1636.434 kb on - strand, within SGL_RS09555 at 1636.461 kb on + strand, within SGL_RS09555 at 1636.489 kb on + strand, within SGL_RS09555 at 1636.535 kb on - strand, within SGL_RS09555 at 1636.558 kb on - strand, within SGL_RS09555 at 1636.568 kb on + strand, within SGL_RS09555 at 1636.576 kb on - strand, within SGL_RS09555 at 1636.864 kb on + strand, within SGL_RS09560 at 1636.864 kb on + strand, within SGL_RS09560 at 1636.872 kb on - strand, within SGL_RS09560 at 1636.990 kb on - strand, within SGL_RS09560 at 1637.051 kb on - strand, within SGL_RS09560 at 1637.133 kb on + strand, within SGL_RS09560 at 1637.133 kb on + strand, within SGL_RS09560 at 1637.160 kb on - strand, within SGL_RS09560 at 1637.165 kb on - strand, within SGL_RS09560 at 1637.221 kb on + strand, within SGL_RS09560 at 1637.307 kb on - strand, within SGL_RS09560 at 1637.391 kb on + strand at 1637.399 kb on - strand at 1637.439 kb on + strand at 1637.452 kb on - strand at 1637.492 kb on - strand at 1637.499 kb on - strand at 1637.519 kb on - strand at 1637.552 kb on + strand at 1637.579 kb on - strand, within SGL_RS09565 at 1637.597 kb on + strand, within SGL_RS09565 at 1638.133 kb on - strand, within SGL_RS09565
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 1,635,782 + +0.6 1,635,789 + +1.3 1,635,790 - +1.0 1,635,797 - +1.0 1,635,797 - +0.8 1,635,985 + SGL_RS09555 0.25 -0.0 1,636,164 - SGL_RS09555 0.44 -0.1 1,636,223 - SGL_RS09555 0.50 +0.7 1,636,248 + SGL_RS09555 0.52 +0.2 1,636,283 + SGL_RS09555 0.56 +1.0 1,636,320 + SGL_RS09555 0.60 -0.1 1,636,328 - SGL_RS09555 0.61 +0.6 1,636,349 - SGL_RS09555 0.63 +1.0 1,636,371 + SGL_RS09555 0.65 +0.5 1,636,392 + SGL_RS09555 0.68 +0.8 1,636,402 + SGL_RS09555 0.69 +0.5 1,636,434 - SGL_RS09555 0.72 +1.0 1,636,434 - SGL_RS09555 0.72 +0.7 1,636,461 + SGL_RS09555 0.75 +0.4 1,636,489 + SGL_RS09555 0.78 +1.0 1,636,535 - SGL_RS09555 0.82 +0.5 1,636,558 - SGL_RS09555 0.85 +0.6 1,636,568 + SGL_RS09555 0.86 +0.9 1,636,576 - SGL_RS09555 0.87 +0.3 1,636,864 + SGL_RS09560 0.21 +1.8 1,636,864 + SGL_RS09560 0.21 +1.3 1,636,872 - SGL_RS09560 0.22 +0.9 1,636,990 - SGL_RS09560 0.38 +1.0 1,637,051 - SGL_RS09560 0.46 +1.5 1,637,133 + SGL_RS09560 0.57 +0.8 1,637,133 + SGL_RS09560 0.57 +1.4 1,637,160 - SGL_RS09560 0.61 +0.9 1,637,165 - SGL_RS09560 0.61 +0.9 1,637,221 + SGL_RS09560 0.69 +0.8 1,637,307 - SGL_RS09560 0.80 +1.1 1,637,391 + -0.0 1,637,399 - +2.0 1,637,439 + +0.8 1,637,452 - +0.5 1,637,492 - +0.5 1,637,499 - +0.5 1,637,519 - +0.2 1,637,552 + +0.1 1,637,579 - SGL_RS09565 0.13 +0.1 1,637,597 + SGL_RS09565 0.15 -0.4 1,638,133 - SGL_RS09565 0.83 +0.1
Or see this region's nucleotide sequence