Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS09235 and SGL_RS09240 are separated by 380 nucleotides SGL_RS09240 and SGL_RS09245 are separated by 47 nucleotides SGL_RS09245 and SGL_RS09250 are separated by 106 nucleotides
SGL_RS09235: SGL_RS09235 - adenine phosphoribosyltransferase, at 1,578,725 to 1,579,243
_RS09235
SGL_RS09240: SGL_RS09240 - restriction endonuclease, at 1,579,624 to 1,580,538
_RS09240
SGL_RS09245: SGL_RS09245 - helix-turn-helix domain-containing protein, at 1,580,586 to 1,580,825
_RS09245
SGL_RS09250: SGL_RS09250 - bile acid:sodium symporter family protein, at 1,580,932 to 1,581,810
_RS09250
Position (kb) 1579
1580
1581 Strain fitness (log2 ratio) -1
0
1 at 1578.859 kb on - strand, within SGL_RS09235 at 1579.089 kb on - strand, within SGL_RS09235 at 1579.113 kb on - strand, within SGL_RS09235 at 1579.145 kb on - strand, within SGL_RS09235 at 1579.150 kb on + strand, within SGL_RS09235 at 1579.252 kb on + strand at 1579.253 kb on + strand at 1579.261 kb on - strand at 1579.293 kb on + strand at 1579.301 kb on - strand at 1579.344 kb on + strand at 1579.352 kb on - strand at 1579.429 kb on - strand at 1579.457 kb on + strand at 1579.556 kb on + strand at 1580.392 kb on - strand, within SGL_RS09240 at 1580.630 kb on - strand, within SGL_RS09245 at 1580.656 kb on + strand, within SGL_RS09245 at 1580.656 kb on + strand, within SGL_RS09245 at 1580.664 kb on - strand, within SGL_RS09245 at 1580.785 kb on + strand, within SGL_RS09245 at 1581.154 kb on + strand, within SGL_RS09250 at 1581.200 kb on + strand, within SGL_RS09250
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 1,578,859 - SGL_RS09235 0.26 +0.3 1,579,089 - SGL_RS09235 0.70 -0.3 1,579,113 - SGL_RS09235 0.75 +0.9 1,579,145 - SGL_RS09235 0.81 -0.0 1,579,150 + SGL_RS09235 0.82 +0.8 1,579,252 + -0.1 1,579,253 + -0.1 1,579,261 - +0.1 1,579,293 + +0.6 1,579,301 - -0.2 1,579,344 + -0.3 1,579,352 - -0.4 1,579,429 - +0.1 1,579,457 + +0.8 1,579,556 + -1.0 1,580,392 - SGL_RS09240 0.84 +0.6 1,580,630 - SGL_RS09245 0.18 +0.3 1,580,656 + SGL_RS09245 0.29 -0.9 1,580,656 + SGL_RS09245 0.29 -0.2 1,580,664 - SGL_RS09245 0.33 +0.0 1,580,785 + SGL_RS09245 0.83 -0.1 1,581,154 + SGL_RS09250 0.25 -0.5 1,581,200 + SGL_RS09250 0.30 -0.1
Or see this region's nucleotide sequence