Experiment: growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt SGL_RS05290 and SGL_RS05295 are separated by 232 nucleotides SGL_RS05295 and SGL_RS05300 are separated by 125 nucleotides SGL_RS05300 and SGL_RS05305 are separated by 214 nucleotides
SGL_RS05290: SGL_RS05290 - NAD-dependent epimerase/dehydratase family protein, at 733,401 to 734,432
_RS05290
SGL_RS05295: SGL_RS05295 - heavy-metal-associated domain-containing protein, at 734,665 to 734,859
_RS05295
SGL_RS05300: SGL_RS05300 - M23 family metallopeptidase, at 734,985 to 736,220
_RS05300
SGL_RS05305: SGL_RS05305 - trans-splicing intein-formed DNA polymerase III subunit alpha C-terminal partner DnaE-C, at 736,435 to 737,811
_RS05305
Position (kb) 734
735
736
737 Strain fitness (log2 ratio) -1
0
1
2 at 734.023 kb on - strand, within SGL_RS05290 at 734.207 kb on + strand, within SGL_RS05290 at 734.721 kb on + strand, within SGL_RS05295 at 734.766 kb on - strand, within SGL_RS05295 at 734.800 kb on + strand, within SGL_RS05295 at 734.801 kb on - strand, within SGL_RS05295 at 734.869 kb on - strand at 734.869 kb on - strand at 734.869 kb on - strand at 734.956 kb on + strand at 734.958 kb on + strand at 735.034 kb on + strand at 735.144 kb on + strand, within SGL_RS05300 at 735.334 kb on + strand, within SGL_RS05300 at 735.525 kb on - strand, within SGL_RS05300 at 735.698 kb on + strand, within SGL_RS05300 at 735.704 kb on + strand, within SGL_RS05300 at 735.812 kb on + strand, within SGL_RS05300 at 735.931 kb on + strand, within SGL_RS05300 at 736.042 kb on + strand, within SGL_RS05300 at 736.059 kb on + strand, within SGL_RS05300
Per-strain Table
Position Strand Gene LocusTag Fraction growth in full media supplemented with 5 .M CuSO4 but diluted to prevent light attenuation remove 734,023 - SGL_RS05290 0.60 +0.6 734,207 + SGL_RS05290 0.78 +0.1 734,721 + SGL_RS05295 0.29 +0.4 734,766 - SGL_RS05295 0.52 -0.6 734,800 + SGL_RS05295 0.69 +1.4 734,801 - SGL_RS05295 0.70 +0.3 734,869 - -0.5 734,869 - +0.5 734,869 - -0.3 734,956 + +1.8 734,958 + +1.3 735,034 + +1.4 735,144 + SGL_RS05300 0.13 +0.1 735,334 + SGL_RS05300 0.28 +0.1 735,525 - SGL_RS05300 0.44 +1.1 735,698 + SGL_RS05300 0.58 +1.0 735,704 + SGL_RS05300 0.58 +1.1 735,812 + SGL_RS05300 0.67 +0.8 735,931 + SGL_RS05300 0.77 +1.1 736,042 + SGL_RS05300 0.86 +1.4 736,059 + SGL_RS05300 0.87 +1.4
Or see this region's nucleotide sequence